Light-Scattering Microscope

Department of Physics and Astronomy, University of Pennsylvania, 209 South 33rd Street, Philadelphia, Pennsylvania 19104, USA.
Applied Optics (Impact Factor: 1.78). 08/1999; 38(19):4151-7. DOI: 10.1364/AO.38.004151
Source: PubMed


A dynamic light scattering (DLS) microscope design capable of simultaneous high-quality imaging and scattering measurements is demonstrated. The design takes advantage of modern optical components to mount the laser source for scattering directly on the microscope, yielding a more robust and easily aligned arrangement that exploits the high quality already incorporated in the microscope. A more straightforward DLS data interpretation is also made possible by collecting the scattered light from the back focal plane of the microscope, ensuring that the scattering vectors are well defined.

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