The Inhibition of Tumor Cell Intravasation and Subsequent Metastasis via Regulation of In Vivo Tumor Cell Motility by the Tetraspanin CD151

Department of Cell Biology, Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Cancer cell (Impact Factor: 23.52). 04/2008; 13(3):221-34. DOI: 10.1016/j.ccr.2008.01.031
Source: PubMed


In vivo tumor cell migration through integrin-dependent pathways is key to the metastatic behavior of malignant cells. Using quantitative in vivo assays and intravital imaging, we assessed the impact of cell migration, regulated by the integrin-associated tetraspanin CD151, on spontaneous human tumor cell metastasis. We demonstrate that promoting immobility through a CD151-specific metastasis blocking mAb prevents tumor cell dissemination by inhibiting intravasation without affecting primary tumor growth, tumor cell arrest, extravasation, or growth at the secondary site. In vivo, this loss of migration is the result of enhanced tumor cell-matrix interactions, promoted by CD151, which prevent dissociation by individual cells and leads to a subsequent inhibition of invasion and intravasation at the site of the primary tumor.

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Available from: Andries Zijlstra
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    • "The initial evidence that CD151 promotes metastasis came from a study showing that a monoclonal antibody with unknown specificity inhibited metastasis by a human epidermoid carcinoma line in vivo. This antibody inhibited cell migration without affecting adhesion or proliferation [47] [48]. Overexpression of CD151 has also been associated with poor prognosis in HCC and potentiates the metastatic behaviour of cancerous cells [49]. "
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