Fate and protective effect of marrow stromal cells after subretinal transplantation

The Experimental Center, The First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China.
Acta Biochimica et Biophysica Sinica (Impact Factor: 2.19). 04/2008; 40(3):202-8. DOI: 10.1111/j.1745-7270.2008.00398.x
Source: PubMed


Engraftment of marrow stromal cells (MSCs) has been proposed as a therapeutic approach for degenerative diseases. In this
study we investigated the fate and dynamic progress of grafted MSCs in living retina with the aim of evaluating the use of
transplanted MSCs to treat retinal degeneration. Approximately 1×105gfp-MSCs in 2 μl phosphate-buffered saline were injected into the subretinal space of adult Sprague-Dawley rats. Two weeks later,
approximately 0.174%±0.082% of the transplanted cells had survived and diffused into the subretinal space. Nine weeks after
transplantation the surviving gfp-MSCs accounted for 0.049%±0.023% of the number of cells injected and were mainly located at the injection site. The same
number of MSCs were transplanted into the left eye subretinal space of 3-week-old hereditary retinal degenerative Royal College
of Surgeons rats, and phosphate-buffered saline was injected into their right eyes as a control. Five weeks after transplantation,
the amount of rudimentary photo-receptors was more significantly increased in grafted eyes than in control eyes. The results
indicated that grafted MSCs could survive and rescue retinal degeneration.

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    ABSTRACT: CORRESPONDENCE TO: Fang-Tian Dong. Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China. To access the differentiation of rat mesenchymal stem cell (MSC) in the microenvironment of retinal degeneration induced by the administration of sodium iodate. In-vitro cultured Lewis rat MSC were injected into the sub-retinal space of NaIO(3) induced retinal degeneration rat eyes (30g/L NaIO(3) 100mg/kg). To observe the trace and differentiation of MSC by immuno-fluorescent method successively in 5 weeks after the surgery. The majority of the transplanted cells stay in retinal pigment epithelium layer and cones & rods layer. From the 2(nd) week after transplantation, the engrafted MSC express PCK and rhodopsin under fluorescent microscope. MSC can survive mainly in the outer layer of retina in the microenvironment of retinal degeneration and differentiate forward the RPE cell and photoreceptor.
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