Article

Fate and protective effect of marrow stromal cells after subretinal transplantation

The Experimental Center, The First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China.
Acta Biochimica et Biophysica Sinica (Impact Factor: 2.19). 04/2008; 40(3):202-8. DOI: 10.1111/j.1745-7270.2008.00398.x
Source: PubMed

ABSTRACT

Engraftment of marrow stromal cells (MSCs) has been proposed as a therapeutic approach for degenerative diseases. In this
study we investigated the fate and dynamic progress of grafted MSCs in living retina with the aim of evaluating the use of
transplanted MSCs to treat retinal degeneration. Approximately 1×105gfp-MSCs in 2 μl phosphate-buffered saline were injected into the subretinal space of adult Sprague-Dawley rats. Two weeks later,
approximately 0.174%±0.082% of the transplanted cells had survived and diffused into the subretinal space. Nine weeks after
transplantation the surviving gfp-MSCs accounted for 0.049%±0.023% of the number of cells injected and were mainly located at the injection site. The same
number of MSCs were transplanted into the left eye subretinal space of 3-week-old hereditary retinal degenerative Royal College
of Surgeons rats, and phosphate-buffered saline was injected into their right eyes as a control. Five weeks after transplantation,
the amount of rudimentary photo-receptors was more significantly increased in grafted eyes than in control eyes. The results
indicated that grafted MSCs could survive and rescue retinal degeneration.

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