CYP450 biosensors based on gold chips for antiepileptic drugs determination

Department of Analytical Chemistry, Faculty of Sciences, University of Burgos, Burgos, Spain.
Biosensors & Bioelectronics (Impact Factor: 6.41). 07/2008; 23(11):1733-7. DOI: 10.1016/j.bios.2008.01.030
Source: PubMed


Three-electrode configuration chips containing a Pt, Au and a screen-printed Ag/AgCl as counter, working and reference electrode, respectively, have been developed. Selective determination of Phenobarbital (PB) has been carried out by Cytochrome P450 2B4 (CYP450) immobilization into a polypyrrole matrix onto the gold working electrode. Chronoamperometric experiments show a PB diffusion coefficient of 2.42x10(-6)cm(2)s(-1), a reproducibility and repeatability in terms of residual standard deviation (RSD) of 13% and 5.51%, respectively, and a limit of detection (LOD) of 0.289 micromol dm(-3) (alpha=beta=0.05) for the developed CYP450-biosensor chip. Its performance has been showed by the determination of PB in pharmaceutical drugs. HPLC has been used as reference technique.

Download full-text


Available from: Javier Gonzalo Ruiz, Aug 28, 2014
  • [Show abstract] [Hide abstract]
    ABSTRACT: Recent advances in nanotechnologies resulted in significant progress in the development of sensor systems based on nanocomposite materials. This review summarizes own and literature data on the sensor system based on hemoproteins which are functionally important for medicine. Special attention is paid to the electrochemical nanobiosensors and principles of functioning of the nanobiosensor medical systems. Key wordshemoproteins-electrochemical biosensors-nanocomposite materials-catalase-peroxidase-cytochrome P450-hemoglobin-myoglobin
    No preview · Article · Mar 2010 · Biochemistry (Moscow) Supplement Series B Biomedical Chemistry
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Recent developments in the bioelectroanalysis of pharmaceutical compounds are reviewed, concentrating particularly on the development of electrode materials and measurement strategies and on their application. The advantages of electroanalytical techniques as alternatives to other analytical procedures such as rapid response, sensitivity and low detection limits are highlighted and illustrated. Particular emphasis is given to carbon-based materials for voltammetric electroanalysis; new potentiometric sensors and electrochemical biosensors are also reviewed.
    Full-text · Article · Mar 2012 · Bioanalytical Reviews
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This work shows an easy and fast electrochemical method for Levetiracetam (LEV) determination, which is a novel antiepileptic. Most of the methods used up to now for its determination required a pre-treatment of the sample. It is shown here that the developed Peroxidase based biosensors avoid this kind of drawbacks. Screen-printed carbon electrodes have been used as transducers for the Peroxidase immobilization by pyrrole electropolymerization. Experimental variables that can affect LEV chronoamperometric response, such as hydrogen peroxide concentration, pH and applied potential, have been optimized in order to perform a selective LEV determination. Under these conditions, the performance of the biosensors has been tested. The residual standard deviation (RSD) of the slopes of different calibration curves was 9.77% (n=4 and alpha=0.05) for the reproducibility and 7.73% (n=4 and alpha=0.05) in the case of the repeatability. An average limit of detection of 9.81x10(-6) M (alpha=beta=0.05) was obtained. The biosensors have been finally applied to the determination of LEV in complex matrices, such as pharmaceutical drugs and spiked human plasma samples, yielding successful results.
    Full-text · Article · Dec 2008 · Bioelectrochemistry (Amsterdam, Netherlands)
Show more