Article

Toxicological EFFECT of ZnO nanoparticles based on bacteria. Langmuir 24: 4140-4144

College of Materials Science and Engineering, Sichuan University, Chengdu, China.
Langmuir (Impact Factor: 4.46). 05/2008; 24(8):4140-4. DOI: 10.1021/la7035949
Source: PubMed

ABSTRACT

Streptococcus agalactiae and Staphylococcus aureus are two pathogenetic agents of several infective diseases in humans. Biocidal effects and cellular internalization of ZnO nanoparticles (NPs) on two bacteria are reported, and ZnO NPs have a good bacteriostasis effect. ZnO NPs were synthesized in the EG aqueous system through the hydrolysis of ionic Zn2+ salts. Particle size and shape were controlled by the addition of the various surfactants. Bactericidal tests were performed in an ordinary broth medium on solid agar plates and in liquid systems with different concentrations of ZnO NPs. The biocidal action of ZnO materials was studied by transmission electron microscopy of bacteria ultrathin sections. The results confirmed that bactericidal cells were damaged after ZnO NPs contacted with them, showing both gram-negative membrane and gram-positive membrane disorganization. The surface modification of ZnO NPs causes an increase in membrane permeability and the cellular internalization of these NPs whereas there is a ZnO NP structure change inside the cells.

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Available from: Di Huang, Sep 10, 2015
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    • "Accumulation of NPs either in the cytoplasm or in the periplasmic region may interact with DNA inducing bacterial death (Raghupathi et al. 2011). In addition, the internalization of ZnO NPs in cells had also been proved to produce significant toxicity to bacteria (Hsiao and Huang 2011; Huang et al. 2008). Although many claims have been published regarding the toxicity mechanism of ZnO NPs, the role of its dissolution has been extensively demonstrated in many studies (Ma et al. 2013; Mudunkotuwa et al. 2011). "
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    • "Due to these wide-ranging applications, the potential effects of ZnO nanoparticles on human and environmental health have also attracted broad attention. Recent studies have shown that ZnO nanoparticles can be toxic to a wide range of biological systems, including human epidermal cells [Sharma et al., 2009], bacteria (Streptococcus agalactiae and Staphylococcus aureus) [Huang et al., 2008], zebra fish (Danio rerio) [Zhu et al., 2008], and mice [Wang et al., 2008]. Toxicity concerns for nanoparticles arise from their smaller size and higher surface-to-volume ratio compared with larger particles, as the smaller size might result in greater particle uptake by cells, and the smaller size and larger accessible surface allows for increased and possibly novel physical and/or chemical interactions with biomolecules inside cells with the potential to disrupt complex membrane and cytoskeletal systems and even DNA replication [Nel et al., 2006]. "
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