Toxicological EFFECT of ZnO nanoparticles based on bacteria. Langmuir 24: 4140-4144

College of Materials Science and Engineering, Sichuan University, Chengdu, China.
Langmuir (Impact Factor: 4.46). 05/2008; 24(8):4140-4. DOI: 10.1021/la7035949
Source: PubMed


Streptococcus agalactiae and Staphylococcus aureus are two pathogenetic agents of several infective diseases in humans. Biocidal effects and cellular internalization of ZnO nanoparticles (NPs) on two bacteria are reported, and ZnO NPs have a good bacteriostasis effect. ZnO NPs were synthesized in the EG aqueous system through the hydrolysis of ionic Zn2+ salts. Particle size and shape were controlled by the addition of the various surfactants. Bactericidal tests were performed in an ordinary broth medium on solid agar plates and in liquid systems with different concentrations of ZnO NPs. The biocidal action of ZnO materials was studied by transmission electron microscopy of bacteria ultrathin sections. The results confirmed that bactericidal cells were damaged after ZnO NPs contacted with them, showing both gram-negative membrane and gram-positive membrane disorganization. The surface modification of ZnO NPs causes an increase in membrane permeability and the cellular internalization of these NPs whereas there is a ZnO NP structure change inside the cells.

Download full-text


Available from: Di Huang, Sep 10, 2015
  • Source
    • "Accumulation of NPs either in the cytoplasm or in the periplasmic region may interact with DNA inducing bacterial death (Raghupathi et al. 2011). In addition, the internalization of ZnO NPs in cells had also been proved to produce significant toxicity to bacteria (Hsiao and Huang 2011; Huang et al. 2008). Although many claims have been published regarding the toxicity mechanism of ZnO NPs, the role of its dissolution has been extensively demonstrated in many studies (Ma et al. 2013; Mudunkotuwa et al. 2011). "
    [Show abstract] [Hide abstract]
    ABSTRACT: In this study, we investigated the antibacterial activity of ZnO nanoparticles (NPs) and Lactobacillus-fermentation liquor (LFL) against two pathogenic bacteria in vitro and in vivo. Bactericidal tests were performed on solid agar plates and quantitative real-time PCR (qPCR), and denaturing gradient gel electrophoresis (DGGE) techniques were used to examine the antibacterial activity of the mixture of ZnO NPs and LFL in vivo. The results showed that the mixture exhibited higher antibacterial activity against Salmonella typhimurium in vitro in comparison with ZnO NPs alone. The results showed that ZnO NPs and LFL significantly enhanced microbial diversity in mouse intestine which suggested a synergistic antibacterial activity against the tested pathogenic bacteria that could be used for the control of the spread and persistence of bacterial infections.
    Full-text · Article · Dec 2015 · Applied Microbiology and Biotechnology
  • Source
    • "Another possible mechanism for the antibacterial activity of CNTs was the oxidative stress. Many nanomaterials could induce ROS generation to inhibit bacterial growth (Huang et al. 2008; Jones et al. 2008; Su et al. 2011). Cellular ROS was determined using a probe of DCFH-DA, which could be hydrolyzed to the nonfluorescent dichlorofluorescein (DCFH) by cellular esterase and further oxidized to the fluorescent dichlorofluorescein (DCF) by cellular ROS. Figure 6a, b shows the generation of cellular ROS induced by different concentrations of CNTs within 30 min and 4 h. "
    [Show abstract] [Hide abstract]
    ABSTRACT: To enrich the understanding on interactions between carbon nanotubes (CNTs) and microbes, the responses of a biphenyl-degrading bacterium to single-walled carbon nanotubes (SWCNTs), multi-walled carbon nanotubes (MWCNTs) and carboxyl single-walled carbon nanotubes (SWCNT-COOHs) were investigated. Electron microscopy, viability test, cellular membrane integrity, and oxidative stress analyses indicated that CNT toxicity was mainly caused by physical piercing. Apart from antibacterial activities, the experimental results showed that CNTs enhanced cell growth and biphenyl degradation at certain concentrations (1.0-1.5 mg/L). The CNTs aggregated and adsorbed cells and biphenyl to form a CNTs-cells-biphenyl coexisting system, thus it created a suitable microenvironment for cell attachment and proliferation where the cells could utilize biphenyl easier for their growth. To the best of our knowledge, this is the first report about CNTs' impact on biodegradation efficacy and growth of aromatic-degrading bacterium.
    Full-text · Article · Oct 2015 · Environmental Science and Pollution Research
    • "Due to these wide-ranging applications, the potential effects of ZnO nanoparticles on human and environmental health have also attracted broad attention. Recent studies have shown that ZnO nanoparticles can be toxic to a wide range of biological systems, including human epidermal cells [Sharma et al., 2009], bacteria (Streptococcus agalactiae and Staphylococcus aureus) [Huang et al., 2008], zebra fish (Danio rerio) [Zhu et al., 2008], and mice [Wang et al., 2008]. Toxicity concerns for nanoparticles arise from their smaller size and higher surface-to-volume ratio compared with larger particles, as the smaller size might result in greater particle uptake by cells, and the smaller size and larger accessible surface allows for increased and possibly novel physical and/or chemical interactions with biomolecules inside cells with the potential to disrupt complex membrane and cytoskeletal systems and even DNA replication [Nel et al., 2006]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: The relationship between particle size and cytogenotoxicity of ZnO particles was systematically studied in vitro using WIL2-NS human lymphoblastoid cells. Before toxicity measurements, the ZnO particles of three different sizes (26 nm, 78 nm, and 147 nm) were well characterized for their physical and chemical properties to ensure that variations in other properties including surface chemistry and particle shape, which also may influence particle toxicity, were minimal. Cell viability testing showed that increasing cytotoxicity was associated with decreasing particle size. Both the dissolution kinetics of ZnO particles in supplemented cell culture medium and the apparent numbers of ZnO particles internalized by cells were size dependent and showed strong correlation with cytotoxicity. Genotoxicity, as measured by micronucleus formation, was significantly enhanced in the presence of the medium-sized and large-sized particles. The observation that necrosis increased with smaller- sized particles but micronuclei were present to a greater extent with larger- sized particles suggests that different mechanisms of cell damage induction or susceptibilities are operating depending on particle size. Environ. Mol. Mutagen., 2015. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.
    No preview · Article · Aug 2015 · Environmental and Molecular Mutagenesis
Show more