Topical isoflavones provide effective photoprotection to skin

ArticleinPhotodermatology Photoimmunology and Photomedicine 24(2):61-6 · May 2008with14 Reads
DOI: 10.1111/j.1600-0781.2008.00329.x · Source: PubMed
Isoflavones, one main group of phytoestrogens, have antioxidative and photoprotective effects in cellular and mouse studies. The aim of this study is to obtain a more comprehensive understanding of the isoflavone-mediated photoprotection with the pig skin model, a more human-resembling model. The pig skin was treated with five well-known isoflavone compounds (genistein, equol, daidzein, biochanin A, and formononetin) and one antioxidant combination solution of 15% vitamin C and 1% vitamin E and 0.5% ferulic acid (CEF) daily for 4 days. Skin was irradiated with solar-simulated UV irradiation, 1 to 5 minimal erythema dose (MED) at 1-MED intervals. Evaluation was carried out 24 h later by colorimeter-measured erythema and sunburn cell numbers. Topical application of 0.5% solutions of three individual phytoestrogens - genistein, daidzein, biochanin A - are better than similar solutions of equol or formononetin in protecting pig skin from solar-simulated ultraviolet (SSUV)-induced photodamage, as measured by sunburn cell formation and/or erythema. However, the protection was less than that provided by a topical combination antioxidant standard containing 15% L-ascorbic acid, 1%alpha-tocopherol, and 0.5% ferulic acid. Isoflavones provide effective photoprotection and are good candidate ingredients for protection against ultraviolet (UV) photodamage.
    • "Neste caso, a radiação UV interage com o ácido trans-urocanico, gerando o oxigênio singlete e promovendo a oxidação de ácidos nucléicos, de proteínas e de lipídeos, resultando em cancer de pele e envelhecimento (Graf, 1992). Recentemente, estudos realizados por Lin et al. (2008), demonstraram que o tratamento do tecido epitelial com uma solução contendo 15% de vitamina C, 1% de vitamina E e 0,5% de ácido ferúlico gera uma fotoproteção bastante efetiva contra os danos causados pela radiação UV. "
    [Show description] [Hide description] DESCRIPTION: The incomplete reduction of oxygen to water during cellular respiration results in the formation of reactive oxygen species (ROS), oxidative compounds that can generate oxidative stress in determined physiological conditions. Considering the major ROS found within the cell, the most studied is hydrogen peroxide (H2O2). In Saccharomyces cerevisiae, the caffeic acid (CA), one of the most important phenolic compounds found in fruits and vegetables, has the ability to protect the cell against oxidative stress and therefore minimize the damage caused by ROS. As consequences of oxidative stress is aging due to damage to key macromolecules. Thus, the purpose of the present study is to understand how different concentrations of CA could act protecting the cells of S. cerevisiae from chronological aging. In this sense, survival curves were obtained for the wild-type S. cerevisiae BY4741 strain grown in complete culture media for 15 days. The culture media were supplemented or not with CA. Moreover, it was improved the Poissoner Quantitative Drop Test (PQDT) to evaluate chronological aging of the same yeast strain in the presence of different concentrations of CA at 25 °C and 37 °C. The collected data indicated that in the presence of CA the survival of yeast cells increased when compared to control assay (without CA), indicating a role of CA in the promotion of antiaging mechanisms.
    Full-text · Research · Jan 2016 · International Journal of Molecular Sciences
    • "Daidzein is a soy isoflavone [chemical structure shown inFigure 2(e)], which is highly soluble in alkaline environments and is part of a group of compounds, called phytoestrogens [89]. It has demonstrated some chemo-protective potential in the skin, since topical application of daidzein in a study resulted in effective photo-protection [90]. In vitro studies showed that daidzein was able to inhibit UVB induced production of hydrogen peroxide within cells and therefore the protection of the keratinocytes. "
    [Show abstract] [Hide abstract] ABSTRACT: Most anti-cancer drugs are derived from natural resources such as marine, microbial and botanical sources. Cutaneous malignant melanoma is the most aggressive form of skin cancer, with a high mortality rate. Various treatments for malignant melanoma are available, but due to the development of multi-drug resistance, current or emerging chemotherapies have a relatively low success rates. This emphasizes the importance of discovering new compounds that are both safe and effective against melanoma. In vitro testing of melanoma cell lines and murine melanoma models offers the opportunity for identifying mechanisms of action of plant derived compounds and extracts. Common anti-melanoma effects of natural compounds include potentiating apoptosis, inhibiting cell proliferation and inhibiting metastasis. There are different mechanisms and pathways responsible for anti-melanoma actions of medicinal compounds such as promotion of caspase activity, inhibition of angiogenesis and inhibition of the effects of tumor promoting proteins such as PI3-K, Bcl-2, STAT3 and MMPs. This review thus aims at providing an overview of anti-cancer compounds, derived from natural sources, that are currently used in cancer chemotherapies, or that have been reported to show anti-melanoma, or anti-skin cancer activities. Phytochemicals that are discussed in this review include flavonoids, carotenoids, terpenoids, vitamins, sulforaphane, some polyphenols and crude plant extracts.
    Full-text · Article · Aug 2014
    • "Our results are consistent with recent studies on the effects exerted by genistein and other isoflavones in combined formulations. Recently, it has been demonstrated that the topical application of solutions containing 0.5% of four individual isoflavones (genistein, daidzein, biochanin A and formononetin) photo-protects pig skin from either UV-induced sunburn cell formation and/or erythema The authors to investigate the mechanism of action, examine ethanolic solutions of isoflavones for their UV absorption and use the erythema response and sunburn cell numbers in pig skin to evaluate the photo-protective effect of isoflavone [22]. In addition, the isoflavone aglycone forms have poor solubility in water and oil; thus, a special galenic mixture is necessary to introduce these isoflavone preparations into cosmetic formulations. "
    [Show abstract] [Hide abstract] ABSTRACT: Isoflavones exist in nature predominantly as glucosides such as daidzin or genistin and are rarely found in their corresponding aglycone forms daidzein and genistein. The metabolism and absorption of isoflavones ingested with food is well documented, but little is known about their use as topical photo-protective agents. The aim of this study was to investigate in a comparative analysis the photo-protective effects of isoflavones in both their aglycone and glucoside forms. In human skin fibroblasts irradiated with 60 mJ/cm2 ultraviolet B (UVB), we measured the expression levels of COX-2 and Gadd45, which are involved in inflammation and DNA repair, respectively. We also determined the cellular response to UVB-induced DNA damage using the comet assay. Our findings suggest that both the isoflavone glucosides at a specific concentration and combination with an aglycone mixture exerted an anti-inflammatory and photo-protective effect that prevented 41% and 71% of UVB-induced DNA damage, respectively. The advantages of using either isoflavone glucosides or an aglycone mixture in applications in the field of dermatology will depend on their properties and their different potential uses.
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