Article

Microevolution of Dengue Viruses Circulating among Primary School Children in Kamphaeng Phet, Thailand

Armed Forces Research Institute of Medical Sciences, 315/6 Rajvithi Road, Bangkok, Thailand 10400.
Journal of Virology (Impact Factor: 4.44). 07/2008; 82(11):5494-500. DOI: 10.1128/JVI.02728-07
Source: PubMed

ABSTRACT

To determine the extent and structure of genetic variation in dengue viruses (DENV) on a restricted spatial and temporal scale,
we sequenced the E (envelope) genes of DENV-1, -2, and -3 isolates collected in 2001 from children enrolled in a prospective
school-based study in Kamphaeng Phet, Thailand, and diagnosed with dengue disease. Our analysis revealed substantial viral
genetic variation in both time and space, with multiple viral lineages circulating within individual schools, suggesting the
frequent gene flow of DENV into this microenvironment. More-detailed analyses of DENV-2 samples revealed strong clustering
of viral isolates within individual schools and evidence of more-frequent viral gene flow among schools closely related in
space. Conversely, we observed little evolutionary change in those viral isolates sampled over multiple time points within
individual schools, indicating a low rate of mutation fixation. These results suggest that frequent viral migration into Kamphaeng
Phet, coupled with population (school) subdivision, shapes the genetic diversity of DENV on a local scale, more so than in
situ evolution within school catchment areas.

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    • "The outbreaks of dengue have been proposed to have begun in Asia, where the greatest number of dengue cases is currently found. Almost all countries in Southeastern Asia (e.g., Indonesia, Cambodia, Laos, Myanmar, Malaysia, Philippines, Thailand, and Vietnam) have documented epidemic dengue outbreaks (Dorji et al., 2009; Gubler, 1998; Holmes et al., 2009; Jarman et al., 2008; Schreiber et al., 2009; Teoh et al., 2010). DENV is a member of the Flavivirus genus belonging to the Flaviviridae family, with a single stranded, non-segmented, positivesense RNA genome of approximately 10.7 kb that encodes a single open reading frame for three structural proteins (C, capsid; prM/M, precursor of membrane; and E, envelope) and seven non-structural proteins (NS1, NS2a, NS2b, NS3, NS4a, NS4b, and NS5) (Chambers et al., 1990). "
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    ABSTRACT: Since 2000, sporadic imported cases of dengue fever were documented almost every year in Yunnan Province, China. Unexpectedly, a large-scale outbreak of dengue virus (DENV) infection occurred fromAugust to December 2013,with 1538 documented cases. In the current study, 81 dengue-positive patient sampleswere collected from Xishuangbanna, the southernmost prefecture of the Yunnan province, and 23 from Dehong, the westernmost prefecture of the Yunnan province. The full-length envelope genes were amplified and sequenced. Phylogenetic analysis revealed that nine strains (39.1%) and 14 strains (60.9%) from the Dehong prefecture were classified as genotype I of DENV-1 and Asian I genotype of DENV-2, respectively. All strains from Xishuangbanna were identified as genotype II of DENV-3. Bayesian coalescent analysis indicates that the outbreak originated from bordering southeastern Asian countries. These three epidemic genotypes were predicted to originate in Thailand and then migrate into Yunnan through different routes.
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    • "evolutionary forces likely act concomitantly to shape the observed genetic diversity of DENV populations. Previous analyses of DENV microevolution in Kamphaeng Phet suggested frequent immigration by human movement and demographic bottlenecks during transmission (Jarman et al. 2008, Rabaa et al. 2013). It is unlikely that Ae. albopictus, a secondary DENV vector found in Thailand, prevented local adaptation because Ae. aegypti is the predominant mosquito vector at our study sites (Chareonviriyaphap et al. 2003, Koenraadt et al. 2008). "

    Full-text · Article · Jan 2016 · Evolutionary Applications
  • Source
    • "The outbreaks of dengue have been proposed to have begun in Asia, where the greatest number of dengue cases is currently found. Almost all countries in Southeastern Asia (e.g., Indonesia, Cambodia, Laos, Myanmar, Malaysia, Philippines, Thailand, and Vietnam) have documented epidemic dengue outbreaks (Dorji et al., 2009; Gubler, 1998; Holmes et al., 2009; Jarman et al., 2008; Schreiber et al., 2009; Teoh et al., 2010). DENV is a member of the Flavivirus genus belonging to the Flaviviridae family, with a single stranded, non-segmented, positivesense RNA genome of approximately 10.7 kb that encodes a single open reading frame for three structural proteins (C, capsid; prM/M, precursor of membrane; and E, envelope) and seven non-structural proteins (NS1, NS2a, NS2b, NS3, NS4a, NS4b, and NS5) (Chambers et al., 1990). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Since 2000, sporadic imported cases of dengue fever were documented almost every year in Yunnan Province, China. Unexpectedly, a large-scale outbreak of dengue virus (DENV) infection occurred from August to December 2013, with 1538 documented cases. In the current study, 81 dengue-positive patient samples were collected from Xishuangbanna, the southernmost prefecture of the Yunnan province, and 23 from Dehong, the westernmost prefecture of the Yunnan province. The full-length envelope genes were amplified and sequenced. Phylogenetic analysis revealed that nine strains (39.1%) and 14 strains (60.9%) from the Dehong prefecture were classified as genotype I of DENV-1 and Asian I genotype of DENV-2, respectively. All strains from Xishuangbanna were identified as genotype II of DENV-3. Bayesian coalescent analysis indicates that the outbreak originated from bordering southeastern Asian countries. These three epidemic genotypes were predicted to originate in Thailand and then migrate into Yunnan through different routes.
    Full-text · Article · Oct 2015 · Infection, genetics and evolution: journal of molecular epidemiology and evolutionary genetics in infectious diseases
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