... The act of inserting needles for the practice of acupuncture can trigger multiple pathways, many of which are predictable from knowledge of biology since the living organism has multiple sensory systems for interacting with the environment and responding to its challenges. Biological effects can occur as a result of touch and pressure [26,27,41,48,60,66,85], pricking and other non-insertion methods [41,65], skin penetration (shallow insertion) [3,39,40,57,78,87], stimulation of underlying structures (deeper insertion) and manipulation of the needle (rotation, lifting-thrusting) [30,31,45,68,71,87]. Many of these pathways have already been demonstrated with regards to acupuncture needling [6, 14-16, 20, 30, 31, 37, 68, 71, 87, 88] and can involve analgesic [14,30,31,68,87], anti-inflammatory [61,89], micro-circulatory [37,39], circulatory [53,82], immunologic [1,62,86], autonomic [49,51,64,78] and modulation of the somatosensory system [5,63] effects. ...