Antihyperglycemic and antioxidative potential of Matricaria chamomilla L. in streptozotocin-induced diabetic rats

Department of Chemistry (Biochemistry Division), Faculty of Sciences and Arts, Afyon Kocatepe University, Afyonkarahisar, Turkey.
Journal of Natural Medicines (Impact Factor: 1.59). 08/2008; 62(3):284-93. DOI: 10.1007/s11418-008-0228-1
Source: PubMed


Plants with antidiabetic activities provide important sources for the development of new drugs in the treatment of diabetes mellitus. In the present study, we investigated possible antihyperglycemic and antioxidative activities of the aerial part of the Matricaria chamomilla L. ethanolic extract (MCE) in streptozotocin (STZ; 70 mg/kg, i.p.)-induced diabetic rats. The following groups were assigned; sham (did not receive any substance), STZ + distilled water (control), STZ + 5 mg/kg glibenclamide, STZ + 20 mg/kg MCE, STZ + 50 mg/kg MCE, STZ + 100 mg/kg MCE. Diabetic rats were treated for 14 days by gavage. Postprandial blood glucose levels, malondialdehyde, reduced glutathione (GSH), nitrate, nitrite, ascorbic acid, retinol, beta-carotene, superoxide dismutase, and catalase levels were measured, and immunohistochemical studies were performed in all of the groups. The obtained data showed that STZ resulted in oxidative stress and affected the antioxidant status. Treatment with different doses of MCE significantly reduced postprandial hyperglycemia and oxidative stress, and augmented the antioxidant system. In histological investigations, MCE treatment protected the majority of the pancreatic islet cells, with respect to the control group. As a result, MCE exhibited significant antihyperglycemic effect and protected beta-cells in STZ-diabetic rats, in a dose-dependent manner, and diminished the hyperglycemia-related oxidative stress.

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    • "Cemek et al. investigated the effects of an ethanolic extract of Matricaria chamomilla in streptozotocin (STZ)-induced diabetic rats, and observed significant reductions in blood glucose after 7 to 14 days with 50-100 mg/kg/d extract administered orally [34]. Kato et al. [35] tested instead of ethanolic, hot water chamomile extracts and major constituents in STZ-induced diabetic rats, and they observed decreased hyperglycaemia with 500 mg/kg/d orally applied extract. "
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    ABSTRACT: Given the significant increases in the incidence of metabolic diseases, efficient strategies for preventing and treating of these common disorders are urgently needed. This includes the development of phytopharmaceutical products or functional foods to prevent or cure metabolic diseases. Plant extracts from edible biomaterial provide a potential resource of structurally diverse molecules that can synergistically interfere with complex disorders. In this study we describe the safe application of ethanolic chamomile (Matricaria recutita) flowers extract (CFE) for the treatment and prevention of type 2 diabetes and associated disorders. We show in vitro that this extract activates in particular nuclear receptor peroxisome proliferator-activated receptor gamma (PPARγ) and its isotypes. In a cellular context, in human primary adipocytes CFE administration (300 µg/ml) led to specific expression of target genes of PPARγ, whereas in human hepatocytes CFE-induced we detected expression changes of genes that were regulated by PPARα. In vivo treatment of insulin-resistant high-fat diet (HFD)-fed C57BL/6 mice with CFE (200 mg/kg/d) for 6 weeks considerably reduced insulin resistance, glucose intolerance, plasma triacylglycerol, non-esterified fatty acids (NEFA) and LDL/VLDL cholesterol. Co-feeding of lean C57BL/6 mice a HFD with 200 mg/kg/d CFE for 20 weeks showed effective prevention of fatty liver formation and hepatic inflammation, indicating additionally hepatoprotective effects of the extract. Moreover, CFE treatment did not reveal side effects, which have otherwise been associated with strong synthetic PPAR-targeting molecules, such as weight gain, liver disorders, hemodilution or bone cell turnover. Taken together, modulation of PPARs and other factors by chamomile flowers extract has the potential to prevent or treat type 2 diabetes and related disorders.
    Full-text · Article · Nov 2013 · PLoS ONE
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    • "Studies with animal model indicate potent anti-inflammatory action,[5] some anti-mutagenic properties,[6] gastro-protective effect,[7] and anxiolytic effects.[8] Chamomile also has acaricidal effect,[9] anti-microbial activity,[10] anti-viral activity,[11] wound healing activity,[12] and anti-hyperglycemic activity.[3] Anti-proliferative and apoptotic effects were observed too.[13] "
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    ABSTRACT: Natural products might alter the labeling of blood constituents with technetium-99m ((99m)Tc) and these results may be correlated with modifications of the shape of the red blood cells (RBC). The biodistribution of radiopharmaceuticals can be also altered. This investigation aimed to determine biological effects of an aqueous extract of chamomile (CE). To study the effect of the CE on the labeling of blood constituents with (99m)Tc, in vitro and in vivo assays were performed. The effect of the CE on the morphology of RBC was observed under light microscope. The images were acquired, processed, and the perimeter/area ratio of the RBC determined. To analyze the effect of the CE on biodistribution of the sodium pertechnetate (Na(99m)TcO4) in Wistar rats, these animals were treated or not with a CE. Na(99m)TcO4 was injected, the rats were sacrificed, the organs were removed, weighted and percentage of radioactivity/gram calculated. In the in vitro experiment, the radioactivity on blood cells compartment and on insoluble fractions of plasma was diminished. The shape and the perimeter/area ratio of the RBC were altered in in vitro assays. An increase of the percentage of radioactivity of Na(99m)TcO4 was observed in stomach after in vivo treatment. These results could be due to substances of the CE or by the products of the metabolism of this extract in the animal organism. These findings are examples of drug interaction with a radiopharmaceutical, which could lead to misdiagnosis in clinical practice with unexpected consequences.
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    • "These reactive compounds can cause peroxidation of lipids, resulting in the formation of hydroperoxy fatty acids and endoperoxides (Pushparaj et al., 2000). Also, Coskun et al. (2005) and Cemek et al. (2008) reported that a considerable necrotic degeneration was observed in the peripheral part of the islets of Langerhans in diabetic rats, Okatomoto (1985) discussed the mechanism of action of STZ by acting as alkylating agent to damage the DNA of the pancreatic beta cell. The author also reported that STZ decomposes to form carbonium ions that alkylate DNA and decrease cellular nicotinamide adenine dinucleotide (NAD) level which may adversely affect the beta cell by interrupting respiratory enzyme activity leading to irreversible cellular necrosis. "
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    Full-text · Article · Oct 2012
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