Basolateral Entry and Release of New and Old World Arenaviruses from Human Airway Epithelia

The Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, USA.
Journal of Virology (Impact Factor: 4.44). 07/2008; 82(12):6034-8. DOI: 10.1128/JVI.00100-08
Source: PubMed


Transmission of arenaviruses from rodent hosts to humans is generally thought to occur through inhalation or ingestion of
dust or droplets containing viral particles. Here we demonstrate that two identified arenavirus receptors, α-dystroglycan
(α-DG) and transferrin receptor 1 (TfR1), are expressed in polarized human airway epithelia. Lymphocytic choriomeningitis
virus strains with high or low α-DG affinity and Junin virus, which binds TfR1, efficiently infected polarized epithelia only
when applied to the basolateral surface or when injury compromised tight junction integrity. Viral egress from infected epithelia
exhibited basolateral polarity. This study demonstrates that respiratory entry of arenaviruses occurs via basolateral receptors.

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    • "Infected rodents excrete the viruses through their urine and saliva (Childs & Peters 1993b). Humans get infected either by cuts or by bites or via inhalation or ingestion of excreta particles (Dylla et al. 2008). People with regular direct or indirect contact with wildlife, agricultural workers (Childs et al. 1995), laboratory workers (Rousseau et al. 1997) and in domestic residences (Kilgore et al. 1995) are particularly susceptible. "
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