Corresponding author: Cheng-Chin Chen, DDS, Ph.D., Department of Dental Hygiene, China Medical University, 91 Hsueh-Shih Road,
Taichung 40402, Taiwan, ROC. Tel: +886-4-2205-3366 ext. 7700, Fax: +886-4-2201-4043, E-Mail: firstname.lastname@example.org
Received: February 14, 2007; Revised: May 10, 2007; Accepted: May 28, 2007.
2007 by The Chinese Physiological Society. ISSN : 0304-4920. http://www.cps.org.tw
Chinese Journal of Physiology 50(6): 308-314, 2007
Effects of Aging and Dietary Antler
Supplementation on the Calcium-Regulating
Hormones and Bone Status in Ovariectomized
Chun-Chi Chen1, Mei-Hui Liu1, Ming-Fu Wang2, and Cheng-Chin Chen3
1Department of Sports, Health and Leisure
Yungta Institute of Technology and Commerce
2Department of Food and Nutrition
Providence University, Taichung
3Department of Dental Hygiene
China Medical University
Taichung, Taiwan, ROC.
This study was conducted to investigate the effects of aging and long-term dietary antler
supplementation on the calcium-regulating hormones and bone status in ovariectomized (Ovx) SAMP8
mice. The female SAMP8 mice were divided into four groups (in each group n = 6), Ovx or sham operated
at the age of 2 months, and fed with 0.2% antler containing diet or control diet from the age of 2.5 months.
The samples were collected at the age of 3, 6, 9, 12, and 15 months, respectively, for physicochemical
analyses, biochemical analyses, and the determination of hormones by radioimmunoassay. The results
showed that plasma calcium (Ca) concentrations were maintained in a narrow range in all groups
throughout the whole experimental period. With aging and/or ovariectomy, plasma parathyroid
hormone (PTH) and 1,25-dihydroxycholecalciferol (1,25-(OH)2-D3) levels increased, and plasma
phosphorus (P) and calcitonin (CT) levels decreased, and the femoral bone densities and Ca contents
increased during the earlier stage, and then decreased gradually in all groups. Plasma PTH and 1,25-
(OH)2-D3 levels in the Ovx mice were significantly higher than those in the intact mice, and plasma P
concentrations, plasma CT levels, femoral bone densities, and femoral Ca contents in the Ovx mice were
significantly lower than those in the intact mice. In addition, the decreases of plasma P levels, plasma
CT levels, femoral bone densities, and femoral Ca contents, and the increases of plasma PTH levels were
moderated by antler administration in both Ovx and intact mice. However, there was no effect of the
dietary antler supplementation on the plasma 1,25-(OH)2-D3 levels in the female mice. It is concluded
that prolonged dietary antler supplementation has important positive effects on bone loss with age and/
or ovarian function deficiency.
Key Words: mice, SAM, ovariectomy, aging, antler, calcium, calcium-regulating hormones, bone
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