Impact of Contact Lens Group on Antifungal Efficacy of Multipurpose Disinfecting Contact Lens Solutions

ArticleinEye & Contact Lens Science & Clinical Practice 34(3):151-9 · June 2008with21 Reads
Impact Factor: 1.47 · DOI: 10.1097/ICL.0b013e31815788a6 · Source: PubMed
Abstract

To evaluate and compare the in vitro effectiveness of multipurpose contact lens solutions (MPDS) to inhibit fungal colonization of Food and Drug Administration (FDA) contact lens groups. Contact lenses from FDA groups I (low water content, nonionic), II (high water content, nonionic), III (low water content, ionic), and IV (high water content, ionic) were placed in sterile contact lens cases with 2 mL of ReNu MultiPlus, ReNu with MoistureLoc, or OPTI-FREE RepleniSH. Each contact lens-multipurpose solution combination was challenged with 0.1 mL of 10(6) colony-forming units/mL of Fusarium oxysporum and incubated at room temperature. Contact lenses and aliquots from phosphate-buffered saline rinse solutions, multipurpose solutions in cases, and lens cases were sampled at 24, 48, and 72 hours, plated on Sabouraud's agar, incubated at room temperature, and observed for growth (i.e., adhesion) for 1 to 7 days. Used multipurpose contact lens solutions met or exceeded the 1-log reduction requirement of the FDA stand-alone test. Viable fungal colonies were recovered from group II lenses after 24 hours of storage in all three multipurpose solutions. Colonization was also detected with at least one contact lens-multipurpose solution pairing from groups I, III, and IV. Fungi were not recovered from lens-solution combinations after 24 hours or from phosphate-buffered saline solutions or lens cases at any period. Antifungal activity of contaminated multipurpose solutions may be insufficient to prevent fungal colonization of contact lens materials despite meeting or exceeding the FDA stand-alone test criteria. Colonized lens may increase the risk of fungal keratitis.

    • "Overall, solutions are more effective against bacteria than they are against fungal organisms or Acanthamoeba. Relatively little attention has been paid to the efficacy of disinfecting solutions against fungi [19,37383940414243. In the U.S., only 1-log reduction of fungal organisms within the recommended disinfection time is required by the FDA to meet the primary Stand Alone Test criteria for contact lens disinfection. "
    [Show abstract] [Hide abstract] ABSTRACT: Introduction: We aimed to investigate the susceptibility of a combined inoculum of Pseudomonas aeruginosa and different fungal strains to 6 soft contact lens disinfectants. Methodology: One corneal isolate of P. aeruginosa and 13 corneal fungal isolates (9 Aspergillus spp, 3 Fusarium spp, 1 Curvularia sp.) were used. The following solutions were tested: Arion Cronos, Complete RevitaLens, Dua Elite, Opti-Free Express, Regard, Oxysept Comfort, and Oxysept Comfort without catalase. The effect of the solutions was assessed on a combined inoculum of P. aeruginosa plus 1 fungal strain. Suspensions of P. aeruginosa and fungi were made in the solutions (1x106 colony-forming units/mL). After 1 hour (Arion Cronos only), 6, 8, and 24 hours, aliquots of suspension were removed and seeded on Luria-Bertani and Sabouraud agar plates. Results: After 6 hours' exposure, all the solutions but Dua Elite and Oxysept Comfort eradicated P. aeruginosa. Conversely, apart from 3% hydrogen peroxide-based Oxysept Comfort without catalase, which eradicated all the fungi tested after 6 hours, all the other solutions were partly ineffective at killing some of the fungal isolates, even after 24 hours' exposure. Conclusions: Most contact lens disinfectants may be ineffective if contact lens care systems become co-contaminated with P. aeruginosa and fungi. In our experiment, only exposure to 3% hydrogen peroxide without neutralizer for at least 6 hours was always able to kill a combined inoculum of P. aeruginosa and different fungal strains.
    Preview · Article · Mar 2013 · The Journal of Infection in Developing Countries
    0Comments 4Citations
    • "MPSs, especially those based on polyhexanide or related active ingredients, were found to be contaminated with Gram-negative bacteria, in particular P. aeruginosa, yeasts (Candida spp.), moulds, and Grampositive bacteria (especially S. marcescens) as well. The high number of contaminated MPSs reported in in-vivo studies [12,25,45,46] and the association of MPSs with an increased risk of CL-related microbial keratitis38394041424344 suggest that MPSs – especially those based on polyhexanide or related active ingredients –should be improved to yield higher antimicrobial and antifungal characteristics, and therefore should be tested under more realistic conditions before being placed on the market. Especially the influence of the tear fluid and its components is an important factor for simulating more realistic conditions. "
    [Show abstract] [Hide abstract] ABSTRACT: Background Contact lens-related infections are often associated with inadequate contact lens hygiene, and therefore, contact lens care products should be able to sufficiently minimise the amount of pathogens that are responsible for these infections. In 2001, the EN ISO 14729 was introduced to ensure adequate disinfection efficacy of contact lens care solutions, but this norm has recently been criticised. Methods In this study, six frequently used contact lens care solutions were retested according to the Stand Alone Test of the EN ISO 14729 (2001). The Stand Alone Test is a quantitative suspension test. In addition, the products were tested in a modified setting adding an organic load. The load was a mixture of human blood serum, lysozyme, and mucine, which resembles tear fluid. Results The criteria of the Stand Alone Test recommended in EN ISO 14729 were only met by Aosept Plus. This 3% hydrogen-peroxide-based contact lens care solution attained a reduction factor of > 5 log units for bacteria and > 4 for fungi in all cases. Two further contact lens care solutions, Blue Vision and Optifree Replenish, met the criteria of a reduction factor of > 3 log units for bacteria and > 1 log unit for fungi, but only in the presence of artificial tear fluid. The three remaining products did not exhibit adequate disinfecting efficacy, at least against one of the tested microorganisms. Conclusions Through the observation that the artificial tear fluid used in this study influences the disinfecting efficacy of contact lens care solutions, especially that of multi-purpose solutions, in a different way than does albumin, mucine, or even the organic load suggested in EN ISO 14729, it becomes obvious that the test conditions in the EN ISO 14729 should be revised in order to create more realistic conditions, e.g., by using a more realistic artificial tear fluid. Furthermore, we suggest adapting the EN ISO 14729 to the European test hierarchy for chemical disinfectants and antiseptics, which consists of three test phases and also requests meeting stricter criteria in order to pass the test. Unless the test conditions guarantee a sufficient reduction of potential pathogens, the risk of contact lens-related microbial keratitis and other infections will remain for the users.
    Full-text · Article · Oct 2012 · BMC Infectious Diseases
    0Comments 6Citations
    • "It must be noted that the challenges with both bacterial and fungal isolates were performed under laboratory conditions and did not allow for variation in performance of disinfection which can occur when this is carried out in real-life situations by contact lens users who have been reported to have poor compliance with lens care routines (Weissman and Mondino, 2002; Yung et al., 2007; ). The presence of contact lenses has been shown to adversely affect disinfecting capacity (Rosenthal et al., 2006; Ide et al., 2008) and the use of old and rarely cleaned or disinfected lens cases will also reduce the efficacy of the solutions due to the formation of biofilm (Anwar et al., 1992; Russell, 2002). The current testing criteria would appear to underestimate the disinfecting requirements for safety of contact lens use. "
    [Show abstract] [Hide abstract] ABSTRACT: Recent outbreaks of microbial keratitis have increased concerns about the efficacy of multipurpose solutions (MPS) against 'real-world' organisms. This study determined, in accordance with FDA/ISO standard methods, the effects of five MPS against clinical isolates and type strains of bacteria, and isolates of fungi from subjects' ocular structures; and of three MPS against environmental fungal isolates. MPS were challenged with bacteria (type strains (ATCC) and clinical isolates of bacterial pathogens obtained from a district hospital laboratory) and with fungal isolates from both the periocular and conjunctival structures and from environmental air. All MPS demonstrated at least a 3-log reduction of challenged cell viability of all bacterial species tested, with the exception of MPS D against a canine infection Staphylococcus aureus isolate. Whilst all MPS tested were able to effect a 1.0-log reduction of viability of Fusarium solani (ATCC 36031), only two MPS had 90% viability reduction against all fungi of human origin and only one of these against all environmental fungal isolates. Effectiveness of these two solutions against fungal isolates compared to the remaining three MPS was found to be statistically significant (p = 0.003). All MPS demonstrated a 99.9% viability reduction against a wide range of bacteria including major ocular pathogens not currently included in the FDA panel. The inability of three MPS to achieve a 90% reduction against fungal isolates is of concern as there has been a recent upsurge in reports of fungal keratitis. We would recommend extension of the current FDA testing panel for MPS to include more fungal isolates.
    Full-text · Article · Jan 2010 · Ophthalmic and Physiological Optics
    0Comments 15Citations
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