Adsorption of Essential Micronutrients by Carbon Nanotubes and the Implications for Nanotoxicity Testing
Division of Engineering Brown University, Providence, RI 02912, USA.Small (Impact Factor: 8.37). 06/2008; 4(6):721-7. DOI: 10.1002/smll.200700754
A study used biochemical profiling techniques and ultraviolet (UV) visible spectroscopy, to demonstrate that single-walled nanotubes (SWNTs) caused dose-dependent adsorption and depletion of more than 14 amino acids and vitamins from RPMI cell culture medium. It was found that HepG2 cells cultured through these techniques, showed significantly reduced viability that can be restored by replenishment of folate. The study demonstrated a new mechanism through which hydrophobic nanotube formulations influenced cell behavior indirectly. The study involved the dispersion of SWNTs in RPMI cell culture medium at different doses and sonicating for 30 minutes, to conduct investigations.