Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad methanolic fruit extract

Department of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra-136119 Haryana, India.
Acta Pharmaceutica (Impact Factor: 0.91). 07/2008; 58(2):215-20. DOI: 10.2478/v10007-008-0008-1
Source: PubMed


Citrullus colocynthis (L.) Schrad. (Cucurbitaceae) is a medicinal plant traditionally used as an abortifacient and to treat constipation, oedema, bacterial infections, cancer and diabetes. Preliminary phytochemical screening of the plant showed the presence of large amounts of phenolics and flavonoids. Subsequent quantification showed the presence of 0.74% (m/m) phenolics (calculated as gallic acid) and 0.13% (m/m) flavonoids calculated as catechin equivalents per 100 g of fresh mass. The presence of phenolic compounds prompted us to evaluate its antioxidant activity. In the present study, methanolic fruit extract of C. colocynthis was screened to evaluate its free radical scavenging effect. The highest antioxidant and free radical scavenging ability of the fruit extract was observed at a concentration of 2500 microg mL(-1).

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    • "The concentration of total phenol compounds in the extract was determined against gallic acid as standard compound (mg of gallic acid equivalent/gm of extract). Total flavonoids (TF) were measured following a previously reported spectrophotometric method [26] against quercetin as a reference standard (mg of quercetin equivalent/gm of extract). The DPPH (2, 2 diphenyl-1-picrylhydrazyl) free radical scavenging activity of C. longa extract was evaluated as described previously [27]. "
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    ABSTRACT: We studied on the effect of Curcuma longa extract on spatial learning-related memory ability of old rats in eight-arm radial maze task. Rats were randomly divided into two groups: one group was orally administered 100 mg/KgBW/day C. longa extract (CLE) dissolved in deionized water and the other group was administered the vehicle alone for 10 weeks. The rats were tested with the partially baited eight-arm radial maze to evaluate two types of spatial memory-related learning ability displayed by reference memory errors (RMEs) and working memory errors (WMEs). Chronic administration of CLE significantly decreased the number of RMEs and WMEs, concurrently with the decreases in the cortico-hippocampal levels of lipid peroxides (LPO) and tumor necrosis factor alpha (TNF-α). In a parallel set of experiments, CLE-pretreated rats of the same age group were subjected to hypoxia-reperfusion injury by carotid artery occlusion to induce oxida-tive stress in the brains in order to examine whether such an in vivo hypoxia-induced oxidative stress could be ameliorated by the extract. Again, the levels of LPO were significantly decreased in the cortico-hippocampal tissues of the CLE-fed hypoxic rats. The histology of the brains also revealed that the CLE-pretreated rats had retained improved cellular integrity. Finally, our results provide the evidence that oral administration of C. longa extract increases the defense against oxidative stress and proinflammatory TNF-α, concurrently with the improvement of memory-related brain cognitive ability of the aged rats.
    Full-text · Article · Sep 2015 · Advances in Alzheimer's Disease
    • "Pharmacological survey on this plant revealed most of the works were done on the fruit pulp of C. colocynthis. As fruit extract exhibited free radical scavenging activity,[9] hypoglycemic activity,[10111213141516] protective ability against oxidative stress in the RBC’s of alloxan induced diabetic rats,[17] antihyperlipidemic and antioxidant actions in alloxan induced diabetic rats,[1819] insulinotropic and immunostimulating activities.[1213202122] Fruits and aerial parts of C. colocynthis also exhibited antimicrobial,[2324] anti-inflammatory activity.[25] "
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    ABSTRACT: Background:Citrullus colocynthis is a folk medicinal plan of United Arab Emirates. Several studies on this plant reported and focused on the biological and toxicological profile of fruits pulp. The present study focused on the antioxidant potency of leaf extract of this plant.Aim:To evaluate the antioxidant and xanthine oxidase (XO) inhibitory activities of C. colocynthis by chemical method.Materials and Methods:Four different solvent extracts (methanol-CCM, methanol: water (1:1)-CCMW, chloroform-CCC and hexane-CCH) of leaves of C. colocynthis were investigated for their free radical scavenging activity using DPPH radical as a substrate, lipid peroxidation (LPO) inhibitory activity using a model system consisting of β-carotene-linoleic acid, superoxide radical scavenging activity (enzymatically/nonenzymatically) and XO inhibitory activity. A dose response curve was plotted for determining SC50 and IC50 values for expressing the results of free radical scavenging activity and XO inhibitory activities respectively.Results:The high polyphenolic content of CCM and CCMW extract showed highest antioxidant activity irrespective the method used for this investigation. The overall results decreased in the order of: CCM > CCMW > CCC > CCH. CCH extract was inactive towards chemically generated superoxide radical and poor DPPH radical scavengers. The results of LPO inhibitory activities of leaves extract (0.1, 0.5 and 1.0 mg/mL) also decreased in the order of: CCM > CCMW > CCC > CCH. Overall 1.0 mg/mL leaves extract showed highest antioxidant potency amongst the studied concentration.Conclusion:CCMW and CCM extract of C. colocynthis exhibited promising antioxidants and XO inhibitory activities.
    No preview · Article · Jul 2014 · Pharmacognosy Research
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    • "The maximum antioxidant activity was observed at a concentration of 3 mg/mL that inhibited 95.862% of DPPH free radical (Figure 1), which is totally in agreement with previous studies. In Kumar et al. study, free radical-scavenging effect of CCT fruit extract increased with increasing concentration, and maximum antioxidant activity was observed at 2.5 mg/mL with the percentage inhibition of 88.0 ± 2.7 [38]. Delazar et al. isolated and identified three types of flavonoid, flavone glucosides, and two cucurbitacin glucosides from the methanol extract of the endemic Iranian species CCT fruits. "
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    ABSTRACT: Possible genoprotective effect of Citrullus colocynthis (L.) (CCT) fruits extract against cyclophosphamide- (CP-)induced DNA damage in mice bone marrow cells was evaluated using micronucleus assay, as an index of induced chromosomal damage. Mice were preadministered with different doses of CCT via intraperitoneal injection for 7 consecutive days followed by injection with CP (70 mg/kg b.w.) 1 hr after the last injection of CCT. After 24 hr, mice were scarified to evaluate the frequency of micronucleated polychromatic erythrocytes (MnPCEs). In addition, the number of polychromatic erythrocytes (PCEs) among 1000 normochromatic erythrocytes (NCEs) per animal was recorded to evaluate bone marrow. Pretreatment with CCT significantly reduced the number of MnPCEs induced by CP in bone marrow cells (P < 0.0001). At 200 mg/kg, CCT had a maximum chemoprotective effect and reduced the number of MnPCEs by 6.37-fold and completely normalized the mitotic activity. CCT also led to marked proliferation and hypercellularity of immature myeloid elements after mice were treated with CP and mitigated the bone marrow suppression. Our study revealed that CCT has an antigenotoxic effect against CP-induced oxidative DNA damage in mice. Therefore, it could be used concomitantly as a supplement to protect people undergoing chemotherapy.
    Full-text · Article · Nov 2013 · The Scientific World Journal
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