Genotypic identification of Cryptosporidium spp. isolated from HIV-infected patients and immunocompetent children of Sao Paulo, Brazil

Instituto Básico de Biociências, Universidade de Taubaté, Avenida Tiradentes 500, Taubaté, SP, Brasil.
Revista do Instituto de Medicina Tropical de São Paulo (Impact Factor: 1.01). 05/2008; 50(3):139-43. DOI: 10.1590/S0036-46652008005000003
Source: PubMed


Cryptosporidium isolates identified in fourteen stool samples, collected from five HIV-infected patients and nine immunocompetent children, living in the State of São Paulo, Brazil, were submitted to a molecular analysis using a nested PCR followed of restriction fragment length polymorphism (RFLP), for genetic characterization. The analysis was based on digestion with RsaI restriction enzyme of a DNA fragment amplified from the Cryptosporidium oocyst wall protein (COWP) gene. Based on this analysis, four samples were identified as Cryptosporidium parvum, eight as Cryptosporidium hominis and two presented a profile that corresponded to Cryptosporidium meleagridis when compared to the standards used in the analysis. The use of molecular methods can be helpful to identify source of infections and risk factors related to Cryptosporidium infection in our communities.

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    • "In the Middle East, C. parvum is the dominant species in humans (Sulaiman et al., 2005; Meamar et al., 2007; Tamer et al., 2007; Al-Brikan et al., 2008; Pirestani et al., 2008). In the rest of the world, especially developing countries, C. hominis is usually the predominant species in humans (Cordova Paz Soldan et al., 2006; Gatei et al., 2006b, 2007, 2008; Muthusamy et al., 2006; Park et al., 2006; Samie et al., 2006; Ajjampur et al., 2007; Bushen et al., 2007; Cama et al., 2007, 2008; Cheun et al., 2007; Hung et al., 2007; Morse et al., 2007; Araujo et al., 2008; Jex et al., 2008). Geographic variations in the distribution of C. parvum and C. hominis can also occur within a country. "
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