Antioxidant and radical scavenging properties of curcumin. Chem Biol Interact

Faculty of Arts and Sciences, Department of Chemistry, Atatürk University, TR-25240 Erzurum, Turkey.
Chemico-Biological Interactions (Impact Factor: 2.58). 08/2008; 174(1):27-37. DOI: 10.1016/j.cbi.2008.05.003
Source: PubMed


Curcumin (diferuoyl methane) is a phenolic compound and a major component of Curcuma longa L. In the present paper, we determined the antioxidant activity of curcumin by employing various in vitro antioxidant assays such as 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH*) scavenging, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, N,N-dimethyl-p-phenylenediamine dihydrochloride (DMPD) radical scavenging activity, total antioxidant activity determination by ferric thiocyanate, total reducing ability determination by the Fe(3+)-Fe(2+) transformation method, superoxide anion radical scavenging by the riboflavin/methionine/illuminate system, hydrogen peroxide scavenging and ferrous ions (Fe(2+)) chelating activities. Curcumin inhibited 97.3% lipid peroxidation of linoleic acid emulsion at 15 microg/mL concentration (20 mM). On the other hand, butylated hydroxyanisole (BHA, 123 mM), butylated hydroxytoluene (BHT, 102 mM), alpha-tocopherol (51 mM) and trolox (90 mM) as standard antioxidants indicated inhibition of 95.4, 99.7, 84.6 and 95.6% on peroxidation of linoleic acid emulsion at 45 microg/mL concentration, respectively. In addition, curcumin had an effective DPPH* scavenging, ABTS*(+) scavenging, DMPD*(+) scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, ferric ions (Fe(3+)) reducing power and ferrous ions (Fe(2+)) chelating activities. Also, BHA, BHT, alpha-tocopherol and trolox, were used as the reference antioxidant and radical scavenger compounds. According to the present study, curcumin can be used in the pharmacological and food industry because of these properties.

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Available from: Ilhami Gülçin, Jun 09, 2014
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    • "Therefore the use of curcumin to induce apoptosis in tumor cells is a potentially promising approach for cancer therapy. For anti-oxidant activity, one study found that curcumin was adept at DPPH scavenging, ABTS scavenging, DMPD scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, ferric ion (Fe 3þ ) reduction, and ferrous ion (Fe 2þ ) chelating [11]. Curcumin has also been shown to have strong antioxidant activity as compared with the standard references (ascorbic acid, BHT and BHA) [12]. "
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    ABSTRACT: The aim of this study was to develop calcium alginate beads containing self-emulsifying curcumin (SE-Cur) for colon targeting. All formulations were prepared by ionotropic gelation and coated with Eudragit® S-100. The beads were characterized for particle size, drug encapsulation and drug release. Encapsulation efficiency was in the range of 85–98%. The formulations containing a mixture of SE-Cur, 2–4% alginate and 0.1 or 0.3 M calcium chloride could prevent early curcumin release in simulated gastric fluid (pH 1.2) and simulated intestinal fluid (pH 6.8) and >60% of the drug was released in simulated colonic fluid (pH 7.4) within 12 h, while the total drug release from the beads containing curcumin powder in all media was only 10–20%. The emulsion droplet sizes in simulated colonic fluid were in the range of 120–202 nm. SE-Cur released from the beads showed cytotoxic ability against the human colon adenocarcinoma cell lines (HT-29) with an IC50 of 10 μg/mL. In addition, the reducing power assay (antioxidant activity) was linearly proportional to the concentration of the SE-Cur released. Our results demonstrate the potential use of SE-Cur loaded alginate beads for the delivery of poorly soluble drugs to the colon.
    Full-text · Article · Oct 2015 · Journal of Drug Delivery Science and Technology
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    • "The major advantage of TAS test is that it determines the antioxidant capacity of all antioxidants in a biological sample and not just the antioxidant capacity of a single compound [27]. The antioxidant effects of CUR in the kidney have been studied by different researchers when nephrotoxic oxidant damage has been induced with vancomycin [28], gentamicin [29] [30] cisplatin [31], and adriamycin [32] but in the absence of CCl 4 . "
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    ABSTRACT: The aim of the present study was to examine the protective effect of curcumin (CUR) on carbon tetrachloride (CCl4)-induced nephrotoxicity to evaluate the detailed mechanisms by which CUR exerts its protective action. Thirty male Wistar-Albino rats weighing 250-300g were randomly divided into three groups: administrations of olive oil (control, po), CCl4 (0.5mg/kg in olive oil sc) every other day for 3 weeks, and CCl4 (0.5mg/kg in olive oil sc) plus CUR (200mg/kg) every day for 3 weeks. Administration of CCl4 significantly (p<0.001) increased the levels of renal function test such as creatinine and blood urea nitrogen (BUN). Furthermore, treatment of CCl4 significantly elevated the oxidant status of renal tissues while decreasing its anti-oxidant status (p<0.001). CUR displayed a renal protective effect as evident by significant decrease in inflammation and apoptosis during histopathological examination. The administration of CCl4 resulted in an increase in malondialdehyde (MDA) production due to an increase in membrane lipid peroxidation; however, the administration of CUR attenuated this, probably via its antioxidant and free radical scavenging properties. The finding of our study indicates that CUR may have an important role to play in protecting the kidney from oxidative insult. Copyright © 2014 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.
    Full-text · Article · Jun 2015 · Pharmacological reports: PR
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    • "The extent of decrease in absorbance at specified wavelength shows potent radical scavenging ability of the antioxidant. Curcumin have already shown free radical scavenging ability against DPPH radical in various In vitro studies (Ak and Gulçin 2008). However, most of the reported literatures have shown effectiveness of curcumin on DPPH scavenging when it is dissolved in organic solvent (Borra et al. 2013). "
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    ABSTRACT: The supramolecular nano-assemblies formed by electrostatic interactions of two oppositely charged lipid and polymer have been made and used as nanocarriers for curcumin to address its bioavailability and solubility issues. These curcumin encapsulated nano-supramolecular assemblies were characterized with respect to their size (dynamic light scattering), morphology (TEM, SEM), zeta potential (Laser Doppler Velocimetry), encapsulation efficiency (EE), curcumin loading (CL) etc. Stability of the nano-assemblies was assessed at different storage times as a function of varying pH and temperature. The physicochemical characterization of nano-assemblies was performed using Fourier Transform Infra Red Spectroscopy (FT-IR) and Differential Scanning Calorimetry (DSC). The in-vitro antioxidant lipid peroxida-tion (TBARS), radical scavenging (DPPH, NO, H 2 O 2 , reducing power) activity assays of powdered curcumin and nano-encapsulated curcumin were performed. It was found that nano-encapsulated curcumin were roughly spherical in shape, presented high positive zeta potential (>30 mV), monodis-perse (polydispersity index <0.3), amorphous in nature, stable in the pH range of 2–6 and have enhanced antioxidant potency in comparison to crystalline curcumin in aqueous media. In conclusion, the curcumin encapsulated nanocarriers system has great potential as functional food ingredient of natural origin.
    Full-text · Article · Feb 2015 · Journal of Food Science and Technology -Mysore-
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