Protective effect of puerarin on diabetic retinopathy in rats

Ophthalmology Hospital, The First Clinical College of Harbin Medical University, Harbin, People's Republic of China.
Molecular Biology Reports (Impact Factor: 2.02). 07/2008; 36(5):1129-33. DOI: 10.1007/s11033-008-9288-2
Source: PubMed


Puerarin is a major active ingredient extracted from the traditional Chinese medicine Ge-gen. The purpose of this study is to investigate the protective effect of puerarin on diabetic retinopathy (DR) and its mechanisms in rats. Seventy-two male Wistar rats were selected and divided at random into three main groups: control group, streptozotocin (STZ) group and puerarin + STZ group. Retinal histopathological observation and electron microscopic examination were performed; retinal vascular endothelial growth factor (VEGF) and hypoxia inducible factor 1 (HIF-1alpha) gene expressions were examined by Reverse transcription-polymerase chain reaction (RT-PCR) analysis. Results showed that the DR induced by STZ was significantly reduced by the treatment of puerarin as judged by the reduction of morphological changes of inner nuclear layer and outer nuclear layer at any time-point. Puerarin regulates expressions of VEGF and HIF-1alpha stimulated by STZ. It was concluded that puerarin exerts significant protective effects against DR in rats, likely regulating angiogenesis factors expressions, and thus may be an effective and promising medicine for treatment of DR.

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    • "It demonstrated that VEGF-induced tight junction protein phosphorylation, tight junction disassembly, and endothelial cell permeability are mediated by PKC. In a study it was concluded that puerarin exerts significant protective effects against DR in rats, likely regulating angiogenesis factors expressions, and thus may be an effective and promising medicine for treatment of DR [104]. "
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    ABSTRACT: The growing number of people with diabetes worldwide suggests that diabetic retinopathy (DR) and diabetic macular edema (DME) will continue to be sight threatening factors. The pathogenesis of diabetic retinopathy is a widespread cause of visual impairment in the world and a range of hyperglycemia-linked pathways have been implicated in the initiation and progression of this condition. Despite understanding the polyol pathway flux, activation of protein kinase C (KPC) isoforms, increased hexosamine pathway flux, and increased advanced glycation end-product (AGE) formation, pathogenic mechanisms underlying diabetes induced vision loss are not fully understood. The purpose of this paper is to review molecular mechanisms that regulate cell survival and apoptosis of retinal cells and discuss new and exciting therapeutic targets with comparison to the old and inefficient preventive strategies. This review highlights the recent advancements in understanding hyperglycemia-induced biochemical and molecular alterations, systemic metabolic factors, and aberrant activation of signaling cascades that ultimately lead to activation of a number of transcription factors causing functional and structural damage to retinal cells. It also reviews the established interventions and emerging molecular targets to avert diabetic retinopathy and its associated risk factors.
    Full-text · Article · Jul 2014 · BioMed Research International
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    • "nd inhibited the mRNA and protein expression of advanced glycation end - product ( AGE ) receptor and vascular endothelial growth factor in retinas ( Chen et al . , 2012b ) . Moreover , the increased mRNA expression of hypoxia - inducible factor - 1α in retina of STZ - stimulated diabetic rats was notably suppressed by puerarin adminis - tration ( Teng et al . , 2009 ) . Marked or complete opacities in the lens were ob - served in STZ - treated rats , whereas only slight lens opacities occurred in rats with peritoneal and peribulbar injection of puerarin . STZ lessened lens epithelial cell ( LEC ) volume with a dense nucleus , induced many bub - bles around the equator area , and stimulated the elev"
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    ABSTRACT: Puerarin is the major bioactive ingredient isolated from the root of the Pueraria lobata (Willd.) Ohwi, which is well known as Gegen (Chinese name) in traditional Chinese medicine. As the most abundant secondary metabolite, puerarin was isolated from Gegen in the late 1950s. Since then, its pharmacological properties have been extensively investigated. It is available in common foods and is used in alternative medicine. It has been widely used in the treatment of cardiovascular and cerebrovascular diseases, diabetes and diabetic complications, osteonecrosis, Parkinson's disease, Alzheimer's disease, endometriosis, and cancer. The beneficial effects of puerarin on the various medicinal purposes may be due to its wide spectrum of pharmacological properties such as vasodilation, cardioprotection, neuroprotection, antioxidant, anticancer, antiinflammation, alleviating pain, promoting bone formation, inhibiting alcohol intake, and attenuating insulin resistance. However, the direct molecular mechanisms and targets remain unclear. This review provides a comprehensive summary of the pharmacological effects of puerarin. Copyright © 2013 John Wiley & Sons, Ltd.
    Full-text · Article · Jul 2014 · Phytotherapy Research
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    • "Pueraria species are popular Chinese herbal medicine with antioxidative and antithrombotic effects. It has been reported that puerarin exhibits effective role in the treatment of hypertension, arteriosclerosis, diabetes mellitus and metabolic syndrome (Luo et al. 2010; Teng et al. 2009; Zhu et al. 2010). Some reports suggested that puerarin can reduce the cellular melanin content by significantly inhibiting cellular tyrosinase activity in vitro (Choi et al. 2010). "
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    ABSTRACT: Puerarin accumulation during shoot cultures in static and liquid medium with or without aeration is described in Pueraria tuberosa (Roxb. ex Willd.) DC. Maximum shoot induction from nodal explants was achieved on Murashige and Skoog's medium supplemented with 1.13 μM TDZ and 0.25 μM IBA after 4 weeks of growth. Puerarin content was higher in shoot cultures grown in liquid medium as compared to static medium. When shoots were grown in growtek bioreactor with different aeration volume, maximum puerarin content (1484 μg/g DW) was recorded with 20 % v/v aeration which was ~2.3 fold higher than puerarin content recorded in control cultures (cultures grown in growtek without aeration). Aeration requirement for organized (shoot) cultures was different for growth and puerarin accumulation.
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