Article

The effect of the platelet concentration in platelet-rich plasma gel on the regeneration of bone. J Bone Joint Surg Br

Department of Orthopaedic Surgery, Nagoya University School of Medicine, Showa-ku Nagoya, Aichi 466-8550, Japan.
The Bone & Joint Journal (Impact Factor: 3.31). 08/2008; 90(7):966-72. DOI: 10.1302/0301-620X.90B7.20235
Source: PubMed

ABSTRACT

The aim of our study was to investigate the effect of platelet-rich plasma on the proliferation and differentiation of rat bone-marrow cells and to determine an optimal platelet concentration in plasma for osseous tissue engineering. Rat bone-marrow cells embedded in different concentrations of platelet-rich plasma gel were cultured for six days. Their potential for proliferation and osteogenic differentiation was analysed. Using a rat limb-lengthening model, the cultured rat bone-marrow cells with platelet-rich plasma of variable concentrations were transplanted into the distraction gap and the quality of the regenerate bone was evaluated radiologically. Cellular proliferation was enhanced in all the platelet-rich plasma groups in a dose-dependent manner. Although no significant differences in the production and mRNA expression of alkaline phosphatase were detected among these groups, mature bone regenerates were more prevalent in the group with the highest concentration of platelets. Our results indicate that a high platelet concentration in the platelet-rich plasma in combination with osteoblastic cells could accelerate the formation of new bone during limb-lengthening procedures.

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Available from: Naoki Ishiguro, Nov 15, 2015
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    • "It has also been shown that proliferation of rat bone marrow cells incorporated within a platelet gel can be enhanced in a dose dependent manner. This suggests that a high platelet concentration in combination with osteoblastic cells within the platelet gel could accelerate the formation of new bone, in vivo [60]. Clinically, the use of mesenchymal stem cells (MSCs) in a platelet gel has potential for periodontal applications by reducing bone defect depth, probing depth, bleeding (upon probing), and tooth mobility [61]. "
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