The effect of the platelet concentration in platelet-rich plasma gel on the regeneration of bone. J Bone Joint Surg Br

Department of Orthopaedic Surgery, Nagoya University School of Medicine, Showa-ku Nagoya, Aichi 466-8550, Japan.
The Bone & Joint Journal (Impact Factor: 3.31). 08/2008; 90(7):966-72. DOI: 10.1302/0301-620X.90B7.20235
Source: PubMed


The aim of our study was to investigate the effect of platelet-rich plasma on the proliferation and differentiation of rat bone-marrow cells and to determine an optimal platelet concentration in plasma for osseous tissue engineering. Rat bone-marrow cells embedded in different concentrations of platelet-rich plasma gel were cultured for six days. Their potential for proliferation and osteogenic differentiation was analysed. Using a rat limb-lengthening model, the cultured rat bone-marrow cells with platelet-rich plasma of variable concentrations were transplanted into the distraction gap and the quality of the regenerate bone was evaluated radiologically. Cellular proliferation was enhanced in all the platelet-rich plasma groups in a dose-dependent manner. Although no significant differences in the production and mRNA expression of alkaline phosphatase were detected among these groups, mature bone regenerates were more prevalent in the group with the highest concentration of platelets. Our results indicate that a high platelet concentration in the platelet-rich plasma in combination with osteoblastic cells could accelerate the formation of new bone during limb-lengthening procedures.

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Available from: Naoki Ishiguro, Nov 15, 2015
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    • "It has also been shown that proliferation of rat bone marrow cells incorporated within a platelet gel can be enhanced in a dose dependent manner. This suggests that a high platelet concentration in combination with osteoblastic cells within the platelet gel could accelerate the formation of new bone, in vivo [60]. Clinically, the use of mesenchymal stem cells (MSCs) in a platelet gel has potential for periodontal applications by reducing bone defect depth, probing depth, bleeding (upon probing), and tooth mobility [61]. "
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    ABSTRACT: Human bone is a tissue with a fairly remarkable inherent capacity for regeneration; however, this regenerative capacity has its limitations, and defects larger than a critical size lack the ability to spontaneously heal. As such, the development and clinical translation of effective bone regeneration modalities are paramount. One regenerative medicine approach that is beginning to gain momentum in the clinical setting is the use of platelet-rich plasma (PRP). PRP therapy is essentially a method for concentrating platelets and their intrinsic growth factors to stimulate and accelerate a healing response. While PRP has shown some efficacy in both in vitro and in vivo scenarios, to date its use and delivery have not been optimized for bone regeneration. Issues remain with the effective delivery of the platelet-derived growth factors to a localized site of injury, the activation and temporal release of the growth factors, and the rate of growth factor clearance. This review will briefly describe the physiological principles behind PRP use and then discuss how engineering its method of delivery may ultimately impact its ability to successfully translate to widespread clinical use.
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    • "It was also recently implied to have antimicrobial properties which also contribute to tissue repair and regeneration.62 It has been demonstrated that the administration of PRP in combination with bone marrow cells during the consolidation phase of distraction osteogenesis enhances the bone healing process.63–67 PRP can also be an effective scaffold to induce osteogenesis. "
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    • "× 10 9 /mL) stimulates BMSC proliferation and osteogenic differentiation. Kawasumi et al.[140]reported that BMSC proliferation and bone formation were more prevalent in the highest concentration of PRP (4.3×10 9 /mL). Arpornmaeklong et al.[141]reported that PRP (3.5×10 9 /ml) had a dose-dependent stimulation of the BMSC proliferation while reducing ALP activity and calcium deposition.Chen et al./mL) show no capability in the mitogenic and osteoinductive stimulation of BMSCs[194]. "
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