Effect of adipose-derived nucleated cell fractions on tendon repair ina collagenase-induced tendonitis model

Comparative Orthopaedics Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853-6401, USA.
American Journal of Veterinary Research (Impact Factor: 1.34). 08/2008; 69(7):928-37. DOI: 10.2460/ajvr.69.7.928
Source: PubMed


To assess the potential of adipose-derived nucleated cell (ADNC) fractions to improve tendon repair in horses with collagenase-induced tendinitis.
8 horses.
Collagenase was used to induce tendinitis in the superficial digital flexor tendon of 1 forelimb in each horse. Four horses were treated by injection of autogenous ADNC fractions, and 4 control horses were injected with PBS solution. Healing was compared by weekly ultrasonographic evaluation. Horses were euthanatized at 6 weeks. Gross and histologic evaluation of tendon structure, fiber alignment, and collagen typing were used to define tendon architecture. Biochemical and molecular analyses of collagen, DNA, and proteoglycan and gene expression of collagen type I and type III, decorin, cartilage oligomeric matrix protein (COMP), and insulin-like growth factor-I were performed.
Ultrasonography revealed no difference in rate or quality of repair between groups. Histologic evaluation revealed a significant improvement in tendon fiber architecture; reductions in vascularity, inflammatory cell infiltrate, and collagen type III formation; and improvements in tendon fiber density and alignment in ADNC-treated tendons. Repair sites did not differ in DNA, proteoglycan, or total collagen content. Gene expression of collagen type I and type III in treated and control tendons were similar. Gene expression of COMP was significantly increased in ADNC-injected tendons.
ADNC injection improved tendon organization in treated tendons. Although biochemical and molecular differences were less profound, tendons appeared architecturally improved after ADNC injection, which was corroborated by improved tendon COMP expression. Use of ADNC in horses with tendinitis appears warranted.

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Available from: Linda A. Dahlgren, Jul 29, 2015
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    • "There was no control of age, sex, or trainer for enrolled animals. The inclusion criteria were an ultrasound evaluation of the cross-sectional area (CSA) of the tendon core lesion ranging between 20 and 50 and, in longitudinal scans, a fiber alignment score (FAS) of 2 (target path 25%–50% parallel) [1]. Cross-sectional area and FAS at the same section of the contralateral tendon have been used as control of the measurements. "
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    • "Every 8th coronal section was selected, and 6 different histologic parameters were semiquantitatively scored from 1 (severe changes) to 4 (normal) according to the criteria defined in Table 2. Tendon tissues were evaluated for the linearity of their fibre structure, the shape of the tendon cells, the density of the tendon cells, inflammation, hemorrhage, and the thickness of the epitenon, modified from Nixon [9]. "
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    • "However, treatment with autologous MSCs has limitations, such as in acute injuries, because expansion of MSCs by culturing takes 10 to 21 days [5], or in elderly patients because there is a decrease in the quantity, proliferation and differentiation potential of MSCs [30]. Nevertheless, adipose-derived nucleated cells have a short interval for isolation of an injectable uncultured cell pool (24 to 48 hours), providing distinct advantages with regard to timeliness compared with an injection of cultured MSCs from other sources [29,31]. "
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