Cell Cycle Regulation by Oncogenic Tyrosine Kinases in Myeloid Neoplasias: From Molecular Redox Mechanisms to Health Implications

Harvard University, Cambridge, Massachusetts, United States
Antioxidants & Redox Signaling (Impact Factor: 7.41). 08/2008; 10(10):1813-48. DOI: 10.1089/ars.2008.2071
Source: PubMed


Neoplastic expansion of myeloid cells is associated with specific genetic changes that lead to chronic activation of signaling pathways, as well as altered metabolism. It has become increasingly evident that transformation relies on the interdependency of both events. Among the various genetic changes, the oncogenic BCR-ABL tyrosine kinase in patients with Philadelphia chromosome positive chronic myeloid leukemia (CML) has been a focus of extensive research. Transformation by this oncogene is associated with elevated levels of intracellular reactive oxygen species (ROS). ROS have been implicated in processes that promote viability, cell growth, and regulation of other biological functions such as migration of cells or gene expression. Currently, the BCR-ABL inhibitor imatinib mesylate (Gleevec) is being used as a first-line therapy for the treatment of CML. However, BCR-ABL transformation is associated with genomic instability, and disease progression or resistance to imatinib can occur. Imatinib resistance is not known to cause or significantly alter signaling requirements in transformed cells. Elevated ROS are crucial for transformation, making them an ideal additional target for therapeutic intervention. The underlying mechanisms leading to elevated oxidative stress are reviewed, and signaling mechanisms that may serve as novel targeted approaches to overcome ROS-dependent cell growth are discussed.

1 Follower
2 Reads
  • Source
    • "Although the precise pathways leading to ROS stress in cancer cells remain unclear. Activation of oncogenes (Ras, Bcr-Abl, c-Myc, etc.), aberrant metabolism (higher metabolism associated with unregulated growth), activation of the ROS-producing enzyme NADPH oxidase, mitochondrial dysfunction, and loss of functional p53 are intrinsic factors known to cause increased ROS production (Bosanquet and Bell 2004; Wu 2006; Brandon et al. 2006; Rodrigues et al. 2008 "
    [Show abstract] [Hide abstract]
    ABSTRACT: There has been little focus on the promising ability of metal-based nanoparticles (NPs) to kill cancer cells while sparing normal cells. Many in vitro and in vivo reports suggest that certain metal-based NPs are able to induce apoptosis and autophagy in cancer cells at specific concentrations that are not significantly toxic to non-cancerous cells. Those NPs are thought to exploit the oxidative stress conditions that prevail in cancer cells, which are largely exhausted of antioxidant ability. This review considers the induction of reactive oxygen species (ROS) by metal-based NPs as a mechanism for the specific killing of cancer cells. The article concomitantly provides a comprehensive description of the important pathways and molecules leading to programmed cell death (PCD), which occurs mainly via apoptosis, autophagy, and necroptosis. The PCD pathways are followed as ROS-burdened cancer cells succumb to ROS-generating metal-based NPs. Exploration of nanotechnology interventions in anticancer therapy demands further research into the mechanism of intracellular induction of ROS by metal-based NPs. Furthermore, the induction of ROS by NPs should be strictly controlled if ROS-based therapy is to become a paradigm in cancer therapy.
    Full-text · Article · Jul 2015 · Archives of Toxicology
  • Source
    • "The involvement of ROS in cancer initiation and progression is now strongly established. Apart from its role as a causative factor in carcinogenesis through ROS-induced carcinogenesis, redox dysregulation contributes to malignant transformation and progression through ROS-mediated carcinogenic signaling and redox modulation of apoptotic and survival pathways [25] [26]. Following early studies that described increased production of ROS including superoxide free radical anions and hydrogen peroxide (H2O2) by human tumor cells[27], recent research supports a causative role of altered redox regulation in the genesis of tumor and has identified numerous cellular sources of ROS production in cancer cells, including overexpression of ROS-generating NOX family members and enhanced electron leakage from the mitochondrial respiratory chain [28] [29] [30] [31] [32]. "

    Full-text · Article · Jan 2015
  • Source
    • "Increased ROS formation due to mitochondrial mutations is caused by impaired electron transfer which results in leakage of electrons and subsequent generation of superoxide radical and other ROS [42]. Other factors implicated in the increased formation of ROS in cancer cells include oncogenic initiation, abnormal metabolism, and enhanced activity of inflammatory cytokines [43, 44]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Cancer cells generate reactive oxygen species (ROS) resulting from mitochondrial dysfunction, stimulation of oncogenes, abnormal metabolism, and aggravated inflammatory activities. Available evidence also suggests that cancer cells depend on intrinsic ROS level for proliferation and survival. Both physiological and pathophysiological roles have been ascribed to ROS which cause lipid peroxidation. In spite of their injurious effects, the ROS and the resulting lipid peroxidation products could be beneficial in cancer treatment. This review presents research findings suggesting that ROS and the resulting lipid peroxidation products could be utilized to inhibit cancer growth or induce cancer cell death. It also underscores the potential of lipid peroxidation products to potentiate the antitumor effect of other anticancer agents. The review also highlights evidence demonstrating other potential applications of lipid peroxidation products in cancer treatment. These include the prospect of lipid peroxidation products as a diagnostic tool to predict the chances of cancer recurrence, to monitor treatment progress or how well cancer patients respond to therapy. Further and detailed research is required on how best to successfully, effectively, and selectively target cancer cells in humans using lipid peroxidation products. This may prove to be an important strategy to complement current treatment regimens for cancer patients.
    Full-text · Article · Dec 2013 · Oxidative Medicine and Cellular Longevity
Show more