Toxicity assessment of the puffer fish Lagocephalus lagocephalus from the Tunisian coast

Article · September 2008with21 Reads
DOI: 10.1016/j.crvi.2008.05.005 · Source: PubMed
Abstract
This study was undertaken to assess the risk of poisoning due to consumption of the puffer fish Lagocephalus lagocephalus collected along the Tunisian coast. Wistar rats were daily intraperitoneally injected, for 10 days, with acidic extracts of liver or flesh (muscles + skin) of L. lagocephalus. Control rats received injections of NaCl (0.9%). No mortality and no evident signs of neurotoxicity were recorded in treated rats. Conversely, treatment led to: (1) diarrhoea and body and organ (liver, kidney) weights loss; (2) oxidative stress evidenced by an increase in lipid peroxidation (TBARS) and conversely a decrease in antioxidant enzyme activities (SOD, catalase, GSH-Px) in tissues (blood cells, liver, kidneys); (3) a decrease in alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities in blood plasma.
    • Various methods have been published on the determination of TTX, among which LC-MS/MS technique most sensitive (Chen and Chou 1998; Jen et al. 2008; Jang et al. 2010; Chulanetra et al. 2011; Lin and Hwang 2012; Rodriguez et al. 2012; Silva et al. 2012). Biological mouse assay has also been used although not sensitive it is used as an assay for many marine toxic compounds (Brillantes et al. 2003; Wu et al. 2005; Sabrah et al. 2006; Noguchi and Arakawa 2008; Saoudi et al. 2008; Simon et al. 2009; Köşker et al. 2015). The content of TTX analogues and isomers of Lagocephalus sp. and L. sceleratus from the Mediterranean Sea were determined by LC-MS/MS (Rodriguez et al. 2012; Kızılkaya 2014 ).
    File · Data · Jan 2017 · Mediterranean Marine Science
    • Various methods have been published on the determination of TTX, among which LC-MS/MS technique most sensitive (Chen and Chou 1998; Jen et al. 2008; Jang et al. 2010; Chulanetra et al. 2011; Lin and Hwang 2012; Rodriguez et al. 2012; Silva et al. 2012). Biological mouse assay has also been used although not sensitive it is used as an assay for many marine toxic compounds (Brillantes et al. 2003; Wu et al. 2005; Sabrah et al. 2006; Noguchi and Arakawa 2008; Saoudi et al. 2008; Simon et al. 2009; Köşker et al. 2015). The content of TTX analogues and isomers of Lagocephalus sp. and L. sceleratus from the Mediterranean Sea were determined by LC-MS/MS (Rodriguez et al. 2012; Kızılkaya 2014 ).
    [Show abstract] [Hide abstract] ABSTRACT: Tetrodotoxin (TTX) and fatty acids contents of five specimens of Lagocephalus sceleratus collected from Antalya on the Turkish Mediterranean coast were studied. TTX was determined by LC-MS/MS analysis in intestines, liver, ovary and muscle. Fatty acids were determined by GC/MS analyses. The oil content was not high (6%) but polyunsaturated n- 3 and n-6 ratios were high.
    Article · Dec 2016 · Mediterranean Marine Science
    • immaculatus) at different concentrations(v/w). respectively(Figure 4). Most of the work on puffer fish showed the liver to be the most toxic part of puffer fish and the muscle being the least toxic (Hashimoto, 1976;Matsui et al., 1981;Nagashima 1999;Saoudi et al.,2008). On the contrary, this study shows that the ovaries can be more toxic than liver because toxin transfer to the skin decreases somewhat on the onset of spawning season and most of the TTX taken up into the liver would be transported to the ovary, presumably with the precursor of yolk proteins that are synthesized in the liver (Wallace 1985;Specker and Sullivan, 1994).
    [Show abstract] [Hide abstract] ABSTRACT: Tetradotoxin poisoning from Puffer fishes (Family Tetreaodontidae) is very common in coastal regions of Asian countries. In the present study, bioassays were conducted to investigate the toxicity of liver and ovaries of puffer fish Arothron immaculatus on Oreochromis mossambica collected from South Andaman. Various concentrations of tissue extract (0.1, 0.2 and 0.3 ml/g body weight) were introduced into the body of the fish by integrating the same in artificial pellet feed as well as through intra-peritoneal injection. The behavioral and morphological changes in fishes were recorded continuously after the introduction of extract till the time of mortality. The tissue extract produced no lethal change on O. mossambica during the feeding experiment with no mortality. The same concentration of toxin when injected intra-peritoneally was proved to be lethal for the experimental organisms triggering a sudden body color change, paralysis followed by death. The introduction of ovary extract was more fatal and the average time for color change was observed at 5.4, 3.1, 1.4 seconds and the average time of death at 93, 72, 70 seconds respectively in three concentrations. Whereas, the intra-peritoneal introduction of liver extract was more lethal as the average time of color change in 0.1, 0.2 and 0.3 ml/g was found to be 77, 87 and 59 seconds and average time of death occurred after 10.22, 4.03, 3.18 minutes respectively. The study suggests that puffer fish, Arothron immaculatus collected from the coast of South Andaman is showing more toxicity in the ovary tissue.
    Full-text · Article · Jun 2015 · Mediterranean Marine Science
    • immaculatus) at different concentrations(v/w). respectively(Figure 4). Most of the work on puffer fish showed the liver to be the most toxic part of puffer fish and the muscle being the least toxic (Hashimoto, 1976; Matsui et al., 1981; Nagashima 1999; Saoudi et al., 2008). On the contrary, this study shows that the ovaries can be more toxic than liver because toxin transfer to the skin decreases somewhat on the onset of spawning season and most of the TTX taken up into the liver would be transported to the ovary, presumably with the precursor of yolk proteins that are synthesized in the liver (Wallace 1985; Specker and Sullivan, 1994).
    [Show abstract] [Hide abstract] ABSTRACT: Tetradotoxin poisoning from Puffer fishes (Family Tetreaodontidae) is very common in coastal regions of Asian countries. In the present study, bioassays were conducted to investigate the toxicity of liver and ovaries of puffer fish Arothron immaculatus on Oreochromis mossambica collected from South Andaman. Various concentrations of tissue extract (0.1, 0.2 and 0.3 ml/g body weight) were introduced into the body of the fish by integrating the same in artificial pellet feed as well as through intra-peritoneal injection. The behavioral and morphological changes in fishes were recorded continuously after the introduction of extract till the time of mortality. The tissue extract produced no lethal change on O. mossambica during the feeding experiment with no mortality. The same concentration of toxin when injected intra-peritoneally was proved to be lethal for the experimental organisms triggering a sudden body color change, paralysis followed by death. The introduction of ovary extract was more fatal and the average time for color change was observed at 5.4, 3.1, 1.4 seconds and the average time of death at 93, 72, 70 seconds respectively in three concentrations. Whereas, the intra-peritoneal introduction of liver extract was more lethal as the average time of color change in 0.1, 0.2 and 0.3 ml/g was found to be 77, 87 and 59 seconds and average time of death occurred after 10.22, 4.03, 3.18 minutes respectively. The study suggests that puffer fish, Arothron immaculatus collected from the coast of South Andaman is showing more toxicity in the ovary tissue.
    Article · Jun 2015 · Mediterranean Marine Science
    • immaculatus) at different concentrations(v/w). respectively(Figure 4). Most of the work on puffer fish showed the liver to be the most toxic part of puffer fish and the muscle being the least toxic (Hashimoto, 1976; Matsui et al., 1981; Nagashima 1999; Saoudi et al., 2008). On the contrary, this study shows that the ovaries can be more toxic than liver because toxin transfer to the skin decreases somewhat on the onset of spawning season and most of the TTX taken up into the liver would be transported to the ovary, presumably with the precursor of yolk proteins that are synthesized in the liver (Wallace 1985; Specker and Sullivan, 1994).
    [Show abstract] [Hide abstract] ABSTRACT: Tetradotoxin poisoning from Puffer fishes (Family Tetreaodontidae) is very common in coastal regions of Asian countries. In the present study, bioassays were conducted to investigate the toxicity of liver and ovaries of puffer fish Arothron immaculatus on Oreochromis mossambica collected from South Andaman. Various concentrations of tissue extract (0.1, 0.2 and 0.3 ml/g body weight) were introduced into the body of the fish by integrating the same in artificial pellet feed as well as through intraperitoneal injection. The behavioral and morphological changes in fishes were recorded continuously after the introduction of extract till the time of mortality. The tissue extract produced no lethal change on O. mossambica during the feeding experiment with no mortality. The same concentration of toxin when injected intra-peritoneally was proved to be lethal for the experimental organisms triggering a sudden body color change, paralysis followed by death. The introduction of ovary extract was more fatal and the average time for color change was observed at 5.4, 3.1, 1.4 seconds and the average time of death at 93, 72, 70 seconds respectively in three concentrations. Whereas, the intra-peritoneal introduction of liver extract was more lethal as the average time of color change in 0.1, 0.2 and 0.3 ml/g was found to be 77, 87 and 59 seconds and average time of death occurred after 10.22, 4.03, 3.18 minutes respectively. The study suggests that puffer fish, Arothron immaculatus collected from the coast of South Andaman is showing more toxicity in the ovary tissue.
    Full-text · Article · May 2015 · Mediterranean Marine Science
    • The oceanic puffer (Lagocephalus lagocephalus) is a circumglobal species, distributed in tropical and sub-tropical waters of the Atlantic, Pacific and Indian Oceans. Although the species has long been known from the Mediterranean (Dulčić & Pallaoro, 2006), it is infrequently encountered or caught.Saoudi et al. (2008)claimed that the species is often collected along the Tunisian coast but, on the contrary, the species is quite rare in Maltese waters and, in fact, the only published record of the oceanic puffer from Maltese waters is that by Lanfranco (1993); Lanfranco (1993) does not, however, specify any details concerning the caught specimens and l
    Full-text · Article · Dec 2013
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