Novel Cytochrome P450, cyp6a17, Is Required for Temperature Preference Behavior in Drosophila

VIB & Katholieke Universiteit Leuven, Belgium
PLoS ONE (Impact Factor: 3.23). 12/2011; 6(12):e29800. DOI: 10.1371/journal.pone.0029800
Source: PubMed


Perception of temperature is an important brain function for organisms to survive. Evidence suggests that temperature preference behavior (TPB) in Drosophila melanogaster, one of poikilothermal animals, is regulated by cAMP-dependent protein kinase (PKA) signaling in mushroom bodies of the brain. However, downstream targets for the PKA signaling in this behavior have not been identified. From a genome-wide search for the genes regulated by PKA activity in the mushroom bodies, we identified the cyp6a17 Cytochrome P450 gene as a new target for PKA. Our detailed analysis of mutants by genetic, molecular and behavioral assays shows that cyp6a17 is essential for temperature preference behavior. cyp6a17 expression is enriched in the mushroom bodies of the adult brain. Tissue-specific knockdown and rescue experiments demonstrate that cyp6a17 is required in the mushroom bodies for normal temperature preference behavior. This is the first study, to our knowledge, to show PKA-dependent expression of a cytochrome P450 gene in the mushroom bodies and its role as a key factor for temperature preference behavior. Taken together, this study reveals a new PKA-Cytochrome P450 pathway that regulates the temperature preference behavior.

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    • "Genes with specific functions in development are noted in orange (Hwn Halloween: Gilbert 2004, Namiki et al. 2005, Rewitz et al. 2006a, nompH1: Willingham and Keil 2004; bb: Rewitz and O'Connor 2011; cutl: Sztal et al. 2012 cutHC: Qiu et al. 2012) those associated with insecticide resistance are in purple (DDT-R; Daborn et al. 2002, Rst(DDT): Amichot et al. 2004, and Rst(luf): Bogwitz et al. 2005) and others that have been the focus of publications are represented by blue lettering (Hrd: Hardstone et al. 2006, lau: Helvig et al. 2004, temp: Kang et al. 2011, agg: Dierick and Greenspan 2006, and SXE1: Fujii et al. 2008). RNAi-L refers to genes shown to be lethal in an RNAi screen of Chung et al. (2009) and RNAi-S are sublethal in that screen. "
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    • "이는 TRPA1을 발현하는 신경의 자극에 의해 중개되며, 이러한 행동은 극단의 위험 온도에 대해서 회피 하는 선천성 행동(Neely et al., 2011)과 구별된 것으로 뇌의 유 병체에서 일어나는 cAMP-dependent protein kinase(PKA)에 의해 조절된다(Hong et al., 2008). 이 PKA에 대한 표적분자를 찾아내기 위해 초파리 게놈 전체를 대상으로 유전자를 추적한 결과 cytochrome P450의 일종인 cyp6a17로서 동정되었으며, 기능적으로 TPB를 유발하는 것으로 증명되었다(Kang et al., 2011) "
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