Borna Disease Virus Infects Human Neural Progenitor Cells and Impairs Neurogenesis

INRA, ANSES, ENVA, UMR 1161, Maisons Alfort, France.
Journal of Virology (Impact Factor: 4.44). 12/2011; 86(5):2512-22. DOI: 10.1128/JVI.05663-11
Source: PubMed


Understanding the complex mechanisms by which infectious agents can disrupt behavior represents a major challenge. The Borna
disease virus (BDV), a potential human pathogen, provides a unique model to study such mechanisms. Because BDV induces neurodegeneration
in brain areas that are still undergoing maturation at the time of infection, we tested the hypothesis that BDV interferes
with neurogenesis. We showed that human neural stem/progenitor cells are highly permissive to BDV, although infection does
not alter their survival or undifferentiated phenotype. In contrast, upon the induction of differentiation, BDV is capable
of severely impairing neurogenesis by interfering with the survival of newly generated neurons. Such impairment was specific
to neurogenesis, since astrogliogenesis was unaltered. In conclusion, we demonstrate a new mechanism by which BDV might impair
neural function and brain plasticity in infected individuals. These results may contribute to a better understanding of behavioral
disorders associated with BDV infection.

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    • "Expression of viral NS1 protein in cells infected at an MOI of 1 was monitored by immunofluorescence (IFA) analysis with murine IgM antibodies directed against the WNV NS1 protein (Millipore) and with a fluorescein isothiocyanate-conjugate of goat anti-murine IgM antibody (Abcys), as described elsewhere [58]. Fluorescence was monitored under a fluorescence microscope equipped with a video documentation system (Zeiss). "
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    • "Chromatin-Associated Life Cycle of Bornavirus of BDV in the brain (Brnic et al., 2012). More importantly, vertical transmission of BDV was demonstrated in both natural and experimental infection, and BDV antigen was detected in decidual cells and endodermal cells of the infected placenta (Hagiwara et al., 2000; Okamoto et al., 2003). "
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