Effects of the interaction of TiO2 nanoparticles with bisphenol A on their physicochemical properties and in vitro toxicity

MOE Key Laboratory of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China.
Journal of hazardous materials (Impact Factor: 4.53). 11/2011; 199-200:426-32. DOI: 10.1016/j.jhazmat.2011.11.040
Source: PubMed


In this paper we evaluated the effects of the interaction of TiO(2) nanoparticles (nano-TiO(2)) with bisphenol A (BPA) on their physicochemical properties and in vitro toxicity in human embryo L-02 hepatocytes. Different concentrations of BPA (0, 0.1, 1, 10 μmol/L) and nano-TiO(2) (0, 0.1, 1, 10mg/L) were mixed to analyze the size distribution, zeta potential, adsorption capacity and uptake of nano-TiO(2), and the toxicity of nano-TiO(2) and BPA in L-02 cells. The addition of BPA to nano-TiO(2) dispersions increased the aggregation level and zeta potential of nano-TiO(2) in all media. Nano-TiO(2) had a similar adsorption capacity in different media, although a higher aggregation level was observed in cell culture medium. Nano-TiO(2), with or without BPA, could enter L-02 cells after 24h exposure. Nano-TiO(2) alone did not induce significant DNA and chromosome damage, but the mixture of nano-TiO(2) and BPA increased toxicity via increasing oxidative stress, DNA double strand breaks and micronuclei formation. The aggregated nano-TiO(2) can enrich BPA effectively. The BPA-bound nano-TiO(2) are proven to be uptaken into nuclei of exposed cells, which may increase intracellular BPA and nano-TiO(2) levels and thus lead to synergistic toxicity. However only small synergic effects were observed at the concentrations of BPA and nano-TiO(2) used in this study.

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    • "These results show that such NPs have the potential to induce cytotoxicity and genotoxicity in cultured WISH cells [38]. In another study, TiO2NPs alone did not induce significant DNA and chromosome damage in human embryo L-02 hepatocytes, but a mixture of TiO2NPs and bisphenol A increased toxicity by increasing oxidative stress, DNA double-strand breaks, and micronuclei formation [39]. In one study Chinese hamster ovary cells appeared to adapt to chronic exposure to TiO2NPs and to detoxify excess ROS, possibly through the upregulation of SOD and by reducing particle uptake [40]. "
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