Proteomic Analysis of Dystrophic Muscle

ArticleinMethods in molecular biology (Clifton, N.J.) 798(1):357-69 · January 2012with5 Reads
Impact Factor: 1.29 · DOI: 10.1007/978-1-61779-343-1_20 · Source: PubMed

    Abstract

    Mass spectrometry-based proteomics had a major impact on the global characterization of skeletal muscles and has decisively enhanced the field of neuromuscular pathology. Proteomic profiling of x-linked muscular dystrophy has identified a large number of new signature molecules involved in fiber degeneration. Here, we describe the difference in-gel electrophoretic analysis of the dystrophic diaphragm muscle from the MDX mouse model of Duchenne muscular dystrophy. This chapter summarizes the various experimental steps involved in muscle proteomics, such as sample preparation, fluorescence labeling, isoelectric focusing, second-dimension slab gel electrophoresis, image analysis, in-gel digestion and electrospray ionization mass spectrometry.