Seroepidemiological study of human metapneumovirus in New Delhi, India

Department of Microbiology, All India Institute of Medical Sciences, New Delhi - 110 029, India.
Indian journal of medical microbiology (Impact Factor: 0.88). 10/2011; 29(4):363-7. DOI: 10.4103/0255-0857.90162
Source: PubMed


There are a few seroepidemiological studies reported on human metapneumovirus (hMPV) as hMPV was only discovered in the year 2001. This respiratory virus has been reported to be ubiquitous and associated with respiratory tract infections in all age groups. The present study aimed at determining the prevalence of antibodies to hMPV in children and adults of 1 month to 55 years of age.
Serum samples from 100 study subjects were tested for hMPV antibody by an in-house ELISA system that used hMPV-infected cell lysate antigen. Result: The prevalence of antibody to hMPV was lowest in children less than 5 years of age (60%) and increased throughout age to > 80%. Similarly, geometric mean titres were 1:180 in children less than 5 years of age and reached a peak of 1:419 in adults over 35 years of age.
The results show that hMPV infection is acquired early in life and re-infection in later life may maintain the seroprevalence and antibody levels in adult population.

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    ABSTRACT: Objectives: To investigate human metapneumovirus (hMPV) pathogens in children with acute lower respiratory tract infection (ALRIs) in Hangzhou, China. Methods: A total of 2911 paediatric patients with ALRIs hospitalised in our unit from January 2011 to December 2011 were enrolled. Their clinical characteristics were recorded and hMPV was detected by direct immunofluorescence. Results: hMPV was found in 70 (2.40%) samples. The positive rate was highest in children aged >1 to 3 years (3.32%), and lowest in children over 3 years (0.63%) with a significant difference among 3 different age groups (χ2=11.795, P=0.003). The positive rate were higher in bronchiolitis and asthma with infection, and lower in pneumonia and bronchitis with a significant difference among 4 different groups (χ2=9.270, P=0.026). The positive rates of hMPV in children with bronchiolitis and asthma with infection were 2.706 (95%CI: 1.057-6.924) and 2.806 (95%CI: 1.095-7.187) folds of that in children with pneumonia. Moreover, the positive rate was highest in spring with a rate of 5.87%, followed by winter (3.30%), autumn (0.44%) and summer (0.36%). It dramatically increased in spring, and peaked on March with a rate of 7.47% while no hMPV positive sample was found from July to September. Conclusions: hMPV is an important cause of ALRIs. hMPV infection commonly occurs in young children, and spring is the peak infection season in Hangzhou, China.
    No preview · Article · Jan 2013 · Hong Kong Journal of Paediatrics
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    ABSTRACT: Background Acute respiratory tract infections (ARTIs) are one of the major causes of morbidity and mortality among young children in developing countries. Information on the incidence of human metapneumovirus (hMPV) and human bocavirus (HBoV) infections in developing countries, especially among rural children, is very limited. Objectives This study was conducted to identify whether these viruses were associated with ARTI among children ≤5 years of age in rural and peri-urban populations in South India. Methods The study was cross-sectional with prospective sample collection. Oropharyngeal swabs were collected from children ≤5 years of age presenting with ARTI. None of the children in this study were known to have any immunosuppressive conditions. The two viruses, hMPV and HBoV, were identified using semi-nested polymerase chain reaction (PCR) assays and one-step PCR assays, respectively. The lower limits of detection of hMPV and HBoV were 6.69 × 105 plasmid copies and 5.77 × 103 plasmid copies, respectively, per 5 μL PCR reaction input. Results The frequency of hMPV infection in children was higher than that of HBoV infection. The different frequencies of hMPV in patients in various age groups with upper and lower respiratory tract infections were compared, and the variance was found to be insignificant. In the 38 children who were hMPV positive, the majority (73.7 %) were from rural communities. The overall hMPV-positive rate was higher in the rural population than in the peri-urban population, but the difference was statistically insignificant. The youngest age at which hMPV-positive status was recorded was 5 months. Conclusion This study demonstrated that hMPV was associated with a significant number (i.e. >10 %) of ARTIs in children in South India, whereas a relatively smaller number of HBoV infections was observed.
    No preview · Article · Apr 2013 · Molecular Diagnosis & Therapy
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    ABSTRACT: We compared antibodies against human metapneumovirus (HMPV) and respiratory syncytial virus (RSV) in children. The antibody nadirs for both viruses were at 3 to 5 months, and the majority of children were seropositive for both by 2 years. There was no significant difference in the kinetics of maternal antibody decline or seroconversion relative to the two viruses.
    Preview · Article · Aug 2013 · Clinical and vaccine Immunology: CVI
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