Identification of a novel single-stranded, circular DNA virus from bovine stool

Department of Veterinary Virology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea.
Journal of General Virology (Impact Factor: 3.18). 11/2011; 93(Pt 3):635-9. DOI: 10.1099/vir.0.037838-0
Source: PubMed


We report the identification of a novel single-stranded, circular DNA virus isolated from bovine stool. The virus, named bovine stool-associated circular DNA virus (BoSCV), has a genome comprising 2600 bases of circular ssDNA, with two putative ORFs encoding replicase and capsid proteins, arranged inversely. The stem-loop structure was located between the 3' ends of the two putative ORFs, as in chimpanzee stool-associated circular virus (ChimpSCV) and unlike other circular DNA viruses, including members of the families Circoviridae, Nanoviridae and Geminiviridae. BoSCV was also genetically similar to ChimpSCV, with approximately 30 % identity in the replicase and capsid proteins. A phylogenetic analysis based on the replicase protein showed that BoSCV and ChimpSCV are in the same clade. A field survey using BoSCV-specific PCRs targeting ORF1 detected BoSCV and BoSCV-like sequences in bovine and porcine stool samples. BoSCV appears to belong to a new genus of circular DNA viruses.

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    • "In particular, the combination of next generation sequencing technologies, shotgun sequencing and sequence independent rolling circle amplification (RCA) have played a key role in novel ssDNA virus identification. Non-invasive faecal sampling of chimpanzees (Blinkova et al., 2010; Li et al., 2010), pigs (Allan & Ellis, 2000; Li et al., 2010; Shan et al., 2011; Sikorski et al., 2013), badgers, pine martens (van den Brand et al., 2012), rodents (Phan et al., 2011), dogs (Kapoor et al., 2012) and cows (Kim et al., 2012) have resulted in the identification of novel ssDNA viruses. "
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