Pfiffner P, Stadler BM, Rasi C, et al. Cross-reactions vs co-sensitization evaluated by in silico motifs and in vitro IgE microarray testing

ArticleinAllergy 67(2):210-6 · November 2011with7 Reads
Impact Factor: 6.03 · DOI: 10.1111/j.1398-9995.2011.02743.x · Source: PubMed


    Using an in silico allergen clustering method, we have recently shown that allergen extracts are highly cross-reactive. Here we used serological data from a multi-array IgE test based on recombinant or highly purified natural allergens to evaluate whether co-reactions are true cross-reactions or co-sensitizations by allergens with the same motifs.
    The serum database consisted of 3142 samples, each tested against 103 highly purified natural or recombinant allergens. Cross-reactivity was predicted by an iterative motif-finding algorithm through sequence motifs identified in 2708 known allergens.
    Allergen proteins containing the same motifs cross-reacted as predicted. However, proteins with identical motifs revealed a hierarchy in the degree of cross-reaction: The more frequent an allergen was positive in the allergic population, the less frequently it was cross-reacting and vice versa. Co-sensitization was analyzed by splitting the dataset into patient groups that were most likely sensitized through geographical occurrence of allergens. Interestingly, most co-reactions are cross-reactions but not co-sensitizations.
    The observed hierarchy of cross-reactivity may play an important role for the future management of allergic diseases.