CCR2 Polymorphism in Chronic Renal Failure Patients Requiring Long-Term Hemodialysis

Department of Medical Genetics, Faculty of Medicine, Cumhuriyet University, Turkey.
Internal Medicine (Impact Factor: 0.9). 01/2011; 50(21):2457-61. DOI: 10.2169/internalmedicine.50.5119
Source: PubMed


A number of chemokines and chemokine receptors are produced by intrinsic renal cells as well as by infiltrating cells during renal inflammation. The CCR2 chemokine receptor mediates leukocyte chemoattraction in the initiation and amplification phase of renal inflammation. The polymorphism, CCR2-V64I, changes valine 64 of CCR2 to isoleucine. We aimed to determine the frequency of CCR2-V64I polymorphism in patients with chronic renal failure requiring long-term hemodialysis.
The PCR-based restriction fragment length polymorphism (PCR-RFLP) technique was used to assess the gene frequencies of CCR2-641 in CRF patients (n=210) and healthy controls (n=139) in the current study.
The frequencies of the CCR2 genotype were 0.68 for V/V, 0.28 for V/I, and 0.4 for I/I in the CRF patients and 0.81 for V/V, 018 for V/I and 0.1 for I/I in healthy controls. The distribution of the CCR2-V64I mutant genotype was significantly different between subjects with CRF and healthy control subjects (X2=7.197 and p=0.027).
We found that the CCR2-V64I polymorphism was significantly high in CRF patients. In addition to the contribution to disease pathogenesis, it was recently found that chemokines have therapeutic importance in chronic renal failure. The frequency of CCR2-V64I and other chemokine and chemokine receptor polymorphisms in renal pathologies must be further investigated in larger study populations and in different renal diseases.

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Available from: Ozturk Ozdemir, Aug 20, 2014
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    • "Different studies have investigated the association of CCR2V64Il with several inflammatory diseases and have reported controversial results. CCR2V64Il is associated with coronary artery disease (CAD) [13] and also chronic renal failure (CRF) [11]; on the other hand some other studies show no association between CCR2V64Il and myasthenia gravis, Parkinson's and Alzheimer diseases [12] [14]. There is only one study about migraine and CCR2V64Il which revealed an association between migraine and CCR2 but the association did not remain after adjustment for multiple testing [15]. "
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