Correlations of follicular fluid oxidative stress biomarkers and enzyme activities with embryo morphology parameters during in vitro fertilization

Department of Obstetrics, Gynecology and Reproductive Sciences, University of California at San Francisco, San Francisco, California, USA.
Fertility and sterility (Impact Factor: 4.59). 12/2011; 96(6):1357-61. DOI: 10.1016/j.fertnstert.2011.09.032
Source: PubMed


To measure antioxidant enzyme activities and lipid peroxidation levels within follicular fluid (FF) and evaluate correlations with early embryo quality.
Individual FF samples were obtained prospectively on the day of oocyte collection and assessed for lipid peroxidation as specific positional isomers of hydroperoxy and hydroxy fatty acids by high-performance liquid chromatography and antioxidant enzyme activities by automated kinetic enzyme assays. Spearman rank correlation coefficients, adjusted for age and day of transfer, were used to assess associations between antioxidant enzymes and lipid peroxidation products and embryo quality using a 1 follicle-1 oocyte/embryo approach. Post hoc power analysis was conducted to help interpret null results.
A university clinic.
Thirty-nine women undergoing IVF.
Embryo cell number and embryo fragmentation score (EFS) at transfer.
No significant correlations between lipid peroxidation derivatives or antioxidant enzyme activities and embryo quality were obtained. Post hoc power analysis indicated possible undetected associations between EFS and 13-hydroxy octadecatrienoic acid and 13-hydroperoxy octadecadieneoic acid.
Our preliminary dataset suggests that most lipid peroxidation products and antioxidant enzyme activities within FF are not associated with the quality of embryos, using EFS and embryo cell number as end points. However, further consideration of associations between EFS and 13-hydroxy octadecatrienoic acid and 13-hydroperoxy octadecadieneoic acid is warranted.

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Available from: Michael S Bloom
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    • "However, debate continues as to whether oocyte potential is adversely influenced by oxidation stress within the follicle . Several studies have focused on the microenvironment surrounding the oocyte and the ROS and antioxidants found in the follicular fluid (FF) (Appasamy et al., 2008; Fujimoto et al., 2011; Oyawoye et al., 2003; Pasqualotto et al., 2004); indeed, an imbalance between ROS and the antioxidant defence system in the FF could be responsible for abnormal oocyte development, causing damage to the DNA, cytoskeleton and cell membrane, which would result in lower egg quality (Paine et al., 2013). "
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    ABSTRACT: Ovarian stimulation is used with IVF/intracytoplasmic sperm injection (ICSI) cycles to obtain multiple oocytes and improve pregnancy rates; however, it also induces perturbation in the oxidant–antioxidant balance leading to oxidation stress. The present study monitored the plasma antioxidant status in women undergoing a long agonist protocol of ovarian stimulation at three different time points: at baseline (T0), after pituitary suppression (T1) and on the day of oocyte retrieval (T2). The antioxidant composition of follicular fluid samples collected on T2 was also evaluated. Significant decreases (P < 0.05) of plasma vitamin C, vitamin E and carotenoids were found between T1 and T2 but not between T0 and T1. At T2, high plasma vitamin E was associated with high numbers of total and mature oocytes retrieved per patient, which, in turn, were favourable for achieving pregnancy. Accordingly, women who became pregnant presented higher vitamin E concentrations both in plasma and FF than those who did not. In conclusion, this study confirmed the occurrence of significant modifications of the plasma antioxidant profile during ovarian stimulation with gonadotrophins; at the same time, it was found that both systemic and follicular antioxidant status may be related to IVF/ICSI outcome.
    Full-text · Article · Jul 2014 · Reproductive biomedicine online
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    • "Several lines of evidence suggest the importance of age in a decrease in the efficiency of the follicular antioxidant defence system (23, 24). Previous studies have revealed an association between reproductive aging to an increase in free radicals (5) and a decrease in catalase in granulosa cells from preovulatory follicles (25, 26). "
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    ABSTRACT: Background: This study evaluated the impact of body mass index (BMI), total calorie intake and physical activity (PA) as energy expenditure related factors on oxidative stress (OS) in follicular fluid (FF). Materials and methods: This prospective study conducted on 219 infertile women. We evaluated patients' BMI, total calorie intake and PA in their assisted reproduction treatment cycles. Malondialdehyde (MDA) and total antioxidant capacity (TAC) in pooled FF at oocyte retrieval were additionally assessed. Results: There was no relation between OS biomarkers to total calorie intake and PA. The TAC levels in FF adjusted for age, duration of infertility, etiology of infertility, number of used gonadotrophin and PA showed a positive relation to BMI (p=0.001). The number of used gonadotrophin and PA had a negative relation to duration of infertility (p=0.03) and anovulation disorder as an etiology of infertility. The MDA level in FF had a positive association with anovulation disorder as the etiology of infertility (p=0.02). MDA in FF was unaffected by BMI. Conclusion: Increasing age, BMI and PA do not affect OS in FF. In women with longtime infertility and those with anovulation disorder as an etiology of infertility, decreased potent antioxidant defense in the follicular microenvironment may contribute to ovarian function. Therefore antioxidant supplements may be beneficial for these groups of women.
    Full-text · Article · Jul 2014 · International journal of fertility & sterility
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    • "Despite our results and others, not all studies in this field have demonstrated a relation between antioxidant defense capacity and oocyte maturation and in regard to this, some researchers have suggested that antioxidants including vitamins A, C and E, despite their antioxidant capacity, may exert their effect on oocyte maturity through other routes (5, 26-28). This possibility may account for lack of relation between oocyte maturity and MDA level, suggesting not all the antioxidants are required as defense mechanism to lipid peroxidation, but the antioxidants are required in the innate process of oocyte maturation, such as glutathione required for sperm decondensation (29). "
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    ABSTRACT: Background: Fat-rich diet may alter oocyte development and maturation and embryonic development by inducing oxidative stress (OS) in follicular environment. Objective: To investigate the relationship between fat intake and oxidative stress with oocyte competence and embryo quality. Materials and Methods: In observational study follicular fluid was collected from 236 women undergoing assisted reproduction program. Malon-di-aldehyde (MDA) levels and total antioxidant capacity (TAC) levels of follicular fluid were assessed as oxidative stress biomarkers. In assisted reproduction treatment cycle fat consumption and its component were assessed. A percentage of metaphase ΙΙ stage oocytes, fertilization rate were considered as markers of oocyte competence and non-fragmented embryo rate, mean of blastomer and good cleavage (embryos with more than 5 cells on 3 days post insemination) rate were considered as markers of embryo quality. Results: The MDA level in follicular fluid was positively related to polyunsaturated fatty acids intake level (p=0.02) and negatively associated with good cleavage rate (p=0.045). Also good cleavage rate (p=0.005) and mean of blastomer (p=0.006) was negatively associated with polyunsaturated fatty acids intake levels. The percentage of metaphase ΙΙ stage oocyte was positively related to the TAC levels in follicular fluid (p=0.046). The relationship between the OS biomarkers in FF and the fertilization rate was not significant. Conclusion: These findings revealed that fat rich diet may induce the OS in oocyte environment and negatively influence embryonic development. This effect can partially be accounted by polyunsaturated fatty acids uptake while oocyte maturation is related to TAC and oocytes with low total antioxidant capacity have lower chance for fertilization and further development.
    Full-text · Article · Dec 2013 · International Journal of Reproductive BioMedicine
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