β-Cell Generation: Can Rodent Studies Be Translated to Humans?

Department of Nephrology, Leiden University Medical Center, Postal Zone C3-P, P.O. Box 9600, 2300 RC Leiden, The Netherlands.
Journal of Transplantation 10/2011; 2011:892453. DOI: 10.1155/2011/892453
Source: PubMed


β-cell replacement by allogeneic islet transplantation is a promising approach for patients with type 1 diabetes, but the shortage of organ donors requires new sources of β cells. Islet regeneration in vivo and generation of β-cells ex vivo followed by transplantation represent attractive therapeutic alternatives to restore the β-cell mass. In this paper, we discuss different postnatal cell types that have been envisaged as potential sources for future β-cell replacement therapy. The ultimate goal being translation to the clinic, a particular attention is given to the discrepancies between findings from studies performed in rodents (both ex vivo on primary cells and in vivo on animal models), when compared with clinical data and studies performed on human cells.

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Available from: Johanne Ellenbroek
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    • "This has led to a large number of clinical studies involving administration of hMSCs. However, only few reports demonstrated robust differentiation of human MSC into insulin-producing cells [17]. Experiments performed in our laboratory employing lentiviral vectors expressing key master transcription factors to force differentiation of hMSC into insulin-producing cells did not yield an efficient protocol for the formation of endocrine-pancreatic cells. "
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