Bioorthogonal Probes for Polo-like Kinase 1 Imaging and Quantification

Center for Systems Biology, Massachusetts General Hospital, Boston, MA 02114, USA.
Angewandte Chemie International Edition (Impact Factor: 11.26). 09/2011; 50(40):9378-81. DOI: 10.1002/anie.201103273
Source: PubMed


Click inside: A nuclear protein target, polo-like kinase 1 (PLK1) was imaged using a biocompatible bioorthogonal ligation between a specific drug and a fluorescent dye in live cells (see picture). Colocalization of the dye and the protein target was confirmed by antibody staining and by expressing a GFP construct of PLK1. The two-step PLK1 imaging procedure was used to quantify PLK1 expression levels in cancer cell lines of various tissue origins.

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