Article

Noninvasive cell-tracking methods

Department of Radiology, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
Nature Reviews Clinical Oncology (Impact Factor: 14.18). 09/2011; 8(11):677-88. DOI: 10.1038/nrclinonc.2011.141
Source: PubMed

ABSTRACT

Cell-based therapies, such as adoptive immunotherapy and stem-cell therapy, have received considerable attention as novel therapeutics in oncological research and clinical practice. The development of effective therapeutic strategies using tumor-targeted cells requires the ability to determine in vivo the location, distribution, and long-term viability of the therapeutic cell populations as well as their biological fate with respect to cell activation and differentiation. In conjunction with various noninvasive imaging modalities, cell-labeling methods, such as exogenous labeling or transfection with a reporter gene, allow visualization of labeled cells in vivo in real time, as well as monitoring and quantifying cell accumulation and function. Such cell-tracking methods also have an important role in basic cancer research, where they serve to elucidate novel biological mechanisms. In this Review, we describe the basic principles of cell-tracking methods, explain various approaches to cell tracking, and highlight recent examples for the application of such methods in animals and humans.

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    • "Near infrared (NIR) fluorescence imaging is emerging as an attractive imaging technique for in vivo inflammation imaging in real time. Although a few magnetic resonance imaging (MRI) [4] and positron emission tomography (PET) [5] probes for inflammation imaging have been developed, fluorescence imaging has the advantages of high sensitivity and resolution, as well as low instrument cost. The main limitation of optical imaging (i.e., limited tissue penetration caused by tissue absorption and scattering ) can be partially resolved by adopting NIR light, which improves tissue penetration and minimizes tissue autofluorescence [6]. "
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    • "Even though, it is one of the most commonly used techniques for immune cell tracking in vivo, allowing whole-body non-invasive tomography. This technique is only useful for preclinical studies in small animals, due to the limits related to the attenuation of light in tissues (Kircher et al., 2011). All near-infrared (NIR) multiphoton microscopy methods are potential techniques for deep tissue imaging but further studies are needed to better characterize the capabilities of these NIRexcitation techniques and background reduction (Joshi et al., 2013). "
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