Article

Cloning and characterization of a maize bZIP transcription factor, ZmbZIP72, confers drought and salt tolerance in transgenic Arabidopsis. Planta

Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Planta (Impact Factor: 3.26). 08/2011; 235(2):253-66. DOI: 10.1007/s00425-011-1496-7
Source: PubMed

ABSTRACT

In plants, the bZIP (basic leucine zipper) transcription factors regulate diverse functions, including processes such as plant development and stress response. However, few have been functionally characterized in maize (Zea mays). In this study, we cloned ZmbZIP72, a bZIP transcription factor gene from maize, which had only one copy in the maize genome and harbored three introns. Analysis of the amino acid sequence of ZmbZIP72 revealed a highly conserved bZIP DNA-binding domain in its C-terminal region, and four conserved sequences distributed in N- or C-terminal region. The ZmbZIP72 gene expressed differentially in various organs of maize plants and was induced by abscisic acid, high salinity, and drought treatment in seedlings. Subcellular localization analysis in onion epidermal cells indicated that ZmbZIP72 was a nuclear protein. Transactivation assay in yeast demonstrated that ZmbZIP72 functioned as a transcriptional activator and its N terminus (amino acids 23-63) was necessary for the transactivation activity. Heterologous overexpression of ZmbZIP72 improved drought and partial salt tolerance of transgenic Arabidopsis plants, as determined by physiological analyses of leaf water loss, electrolyte leakage, proline content, and survival rate under stress. In addition, the seeds of ZmbZIP72-overexpressing transgenic plants were hypersensitive to ABA and osmotic stress. Moreover, overexpression of ZmbZIP72 enhanced the expression of ABA-inducible genes such as RD29B, RAB18, and HIS1-3. These results suggest that the ZmbZIP72 protein functions as an ABA-dependent transcription factor in positive modulation of abiotic stress tolerance and may be a candidate gene with potential application in molecular breeding to enhance stress tolerance in crops.

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    • "TabZIP60 35S COE Arabidopsis thaliana Drought, cold, salinity No phenotypic alteration [87] ThbZIP1 35S COE Nicotiana tabacum Salinity No phenotypic alteration [88] WABI5 35S COE Nicotiana tabacum Drought, cold osmosis No information [80] ZmABP9 35S COE Arabidopsis thaliana Drought, cold salinity No information [56] ZmbZIP72 35S COE Arabidopsis thaliana Drought, salinity No phenotypic alteration [88] B ZmbZIP60 35S COE Arabidopsis thaliana Drought, salinity Early bolting [88] C GmbZIP62 35S COE Glycine max Cold, salinity No information [89] OsbZIP33 Ai AIE 4 Oryza sativa Drought No information [90] OsbZIP52 35S COE Oryza sativa Drought, cold No phenotypic alteration [91] D GmbZIP132 35S COE Glycine max Salinity No information [92] G GmbZIP78 35S COE Glycine max Salinity, cold No information [93] MsZIP MD18 COE Nicotiana tabacum Drought, salinity No information [94] OsbZIP45 PGD1 COE Oryza sativa Drought No information [83] S GmbZIP44 35S COE Glycine max Salinity, cold No information [93] "
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