Validation of a Diagnostic Microarray for Human Papillomavirus: Coverage of 102 Genotypes

Department of Dermatology, University of California San Francisco, San Francisco, CA 94143, USA.
Journal of nucleic acids 05/2011; 2011:756905. DOI: 10.4061/2011/756905
Source: PubMed


Papillomaviruses have been implicated in a variety of human diseases ranging from common warts to invasive carcinoma of the anogenital mucosa. Existing assays for genotyping human papillomavirus are restricted to a small number of types. Here, we present a comprehensive, accurate microarray strategy for detection and genotyping of 102 human papillomavirus types and validate its use in a panel of 91 anal swabs. This array has equal performance to traditional dot blot analysis with the benefits of added genotype coverage and the ability to calibrate readout over a range of sensitivity or specificity values.

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Available from: Eric Dybbro, Mar 05, 2015
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    • "Examples of this include a biochip for monitoring six sexually transmitted pathogens [2], and one that specifically detects point mutations in the gyrA and parC genes of Neisseria gonorrhoeae that are involved in the formation of ciprofloxacin resistance [3]. A multi-target biochip is ideal for detecting a set of pathogens that cause similar symptoms and co-infections, or that are harmful to the same extent, whether they are encountered singularly or all together [4], [5]. Evaluating multi-target probes can be time consuming, necessitating the need to develop a way of processing a large number of probes simultaneously. "
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    ABSTRACT: BACKGROUND: The role of human papillomavirus (HPV) in the induction and maintenance of cervical, anogenital, and some oropharyngeal carcinomas is well recognized, but its role in cutaneous squamous cell carcinoma (SCC) remains to be elucidated. HPV is thought to act as a possible cocarcinogen in the development of SCC. OBJECTIVE: To review the literature assessing the correlation between and possible causation of HPV and cutaneous SCC in immunocompetent and immunocompromised populations. METHODS: We reviewed HPV sampling and detection methods, epidemiologic studies examining HPV carriage in immunocompetent and immunosuppressed individuals, and evidence asserting an association between HPV and cutaneous SCC. RESULTS: Although an abundant body of evidence points toward a link between HPV and cutaneous SCC, many studies indicate otherwise. Recent studies have focused on viral activity in addition to DNA presence. CONCLUSION: The possibility exists that HPV may play a role in the induction but not maintenance of cutaneous SCC.
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