A 2D gel reference map of the basic human heart proteome
UCD Conway Institute of Biomolecular and Biomedical Research, School of Medicine and Medical Science, University College Dublin, Dublin, Ireland. Proteomics
(Impact Factor: 3.81).
09/2011; 11(17):3582-6. DOI: 10.1002/pmic.201000182
We have undertaken the identification of basic proteins (pH 6-11) of the human heart using 2-DE. Tissue from the left ventricle of human heart was lysed and proteins were separated in the first dimension on pH 6-11 IPG strips using paper-bridge loading followed by separation on 12% SDS polyacrylamide gels in the second dimension. Proteins were then identified by mass spectrometry and analysed using Proline, a proteomic data analysis platform that was developed in-house. The proteome map contains 176 identified spots with 151 unique proteins and has been made available as part of the UCD-2DPAGE database at http://proteomics-portal.ucd.ie:8082. The associated mass spectrometry data have been submitted to PRIDE (Accession number ♯10098). This reference map, and the other heart reference maps available through the UCD-2DPAGE database, will aid further proteomic studies of heart diseases such as dilated cardiomyopathy and ischaemic heart disease.
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Available from: Ashling Holland
- "Proteomic methods that involve gel electrophoresis are highly suitable for the analysis of contractile proteins, regulatory proteins, metabolic enzymes, metabolite transporters, and molecular chaperones . Two-dimensional gel electrophoresis can conveniently separate cardiac proteins in the range of approximately 10 kDa to 200 kDa and isoelectric points ranging from pH3 to pH11 [117, 118, 120]. Combinations of isoelectric focusing with narrow- or wide-range immobilised pH gradients, native gel electrophoresis, nonreducing gel electrophoresis, and reducing gel electrophoresis can be used for various two-dimensional applications [137–140]. "
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Available from: Yueshuai Guo
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