Non-invasive monitoring of hepatocellular carcinoma in transgenic mouse with bioluminescent imaging

Molecular Imaging Research Center, Korea Institute of Radiological and Medical Sciences (KIRAMS), Seoul 139-706, Republic of Korea.
Cancer letters (Impact Factor: 5.62). 11/2011; 310(1):53-60. DOI: 10.1016/j.canlet.2011.06.013
Source: PubMed


A small animal imaging system for hepatocellular carcinoma (HCC)-specific reporter gene expression will enable monitoring of carcinogenesis or therapeutic intervention in vivo. Transgenic mouse was developed in which firefly luciferase (fLuc) expression was controlled by the AFP enhancer/promoter. The bioluminescent signals of the transgenic neonates were strong at their liver region and decreased after birth. Bioluminescent imaging (BLI) of a transgenic mouse treated with N-nitrosodiethylamine revealed distinct fLuc activity in the liver and an increased pattern with time. The transgenic mouse model can be used to monitor AFP producing HCC by a chemical carcinogen in a live animal by BLI.

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Available from: Gi Jeong Cheon, May 03, 2014
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    • "In addition, fluorescent dyes and quantum particles can also be used in these studies. FMI can also be used in transgenic detection [94, 95], and new probes for molecular imaging can be developed to monitor the transgene expression as well as the activity and function of endogenous genes. In addition, FMI can also be used to evaluate the tissue-specificity and inducibility of promoters and enhancers. "
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