In vitro evaluation on the antioxidant capacity of triethylchebulate, an aglycone from Terminalia chebula Retz fruit

Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing.
Indian Journal of Pharmacology (Impact Factor: 0.69). 05/2011; 43(3):320-3. DOI: 10.4103/0253-7613.81508
Source: PubMed


To evaluate the antioxidant and free-radical scavenging activities of triethylchebulate (TCL), an aglycone isolated from the fruit of Terminalia chebula Retz.
Microsomes, mitochondria and red blood cells (RBCs) were isolated from rat liver. The antioxidant capacities were evaluated by determining the inhibitory effects of TCL on lipid peroxidation, hydrogen peroxide (H(2)O(2))-induced RBCs hemolysis and RBCs autoxidative hemolysis. The free-radical scavenging activities were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and 2´,7´-dichlorodihydrofluorescin diacetate (DCFH(2)-DA) assay.
TCL significantly inhibited FeSO(4)/Cys-induced microsomes lipid peroxidation and protected both H(2)O(2-)-induced RBCs hemolysis and RBCs auto-hemolysis in a dose-dependent manner. Furthermore, TCL demonstrated potent DPPH free-radical scavenging ability with IC(50) at 2.4×10(-5) M. In addition, TCL also moderately suppressed azide-induced mitochondria ROS formation.
These results demonstrated that TCL was a strong antioxidant and free-radical scavenger, which might contribute to the anti-oxidative ability of Terminalia chebula Retz.

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    • "Strong antioxidant activity of aqueous extract of T. chebula was observed by studying the inhibition of radiation induced lipid peroxidation in rat liver microsomes at different doses [28] , and methanolic extract was also found to inhibit lipid peroxide formation and to scavenge hydroxyl and superoxide radicals in vitro [29] . Acetone extract has stronger antioxidant activity than alphatocopherol and HPLC analysis with diode array detection indicated the presence of hydroxybenzoic acid derivatives, hydroxycinnamic acid derivatives, flavonol aglycones and their glycosides, as main phenolic compounds [30] "
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    ABSTRACT: Medicinal plants are part and parcel of human society to combat diseases from the dawn of civilization. Terminalia chebula Retz. (Fam. Combretaceae), is called the 'King of Medicine' in Tibet and is always listed at the top of the list of 'Ayurvedic Materia Medica' because of its extraordinary power of healing. The whole plant possesses high medicinal value and traditionally used for the treatment of various ailments for human beings. Some of the folklore people used this plant in the treatment of asthma, sore throat, vomiting, hiccough, diarrhea, dysentery, bleeding piles, ulcers, gout, heart and bladder diseases. The plant has been demonstrated to possess multiple pharmacological and medicinal activities, such as antioxidant, antimicrobial, antidiabetic, hepatoprotective, anti-inflammatory, antimutagenic, antiproliferative, radioprotective, cardioprotective, antiarthritic, anticaries, gastrointestinal motility and wound healing activity. But no systematic updated information on the therapeutic effectiveness of Terminalia chebula, a popular herbal remedy in India and South-East Asia has so far been reported. This review highlights an updated information particularly on the phytochemistry and various pharmacological and medicinal properties of Terminalia chebula Retz. and some of its isolated compounds, along with their safety evaluation. This may provide incentive for proper evaluation of the plant as medicinal agent against the human diseases and also to bridge the lacunae in the existing literature and future scope which may offer immense opportunity for researchers engaged in validation of the traditional claims and development of safe and effective botanical medicine.
    Full-text · Article · Mar 2013 · Asian Pacific Journal of Tropical Biomedicine
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    ABSTRACT: Medicinal plants have been considered valuable and cheap source of unique phytoconstituents which are used extensively in the development of drugs against various diseases. A large proportion of the world population, especially in the developing countries relies mainly on the traditional system of medicine. The use of plants and plant products in medicines is getting popularized because the herbal medicines are cheap and have natural origin with higher safety margins and lesser or no side effects. Terminalia chebula Retz. (T. chebula) belongs to the family Combretaceae and is one of the most important medicinal plants used in medicines of ayurveda, siddha, unani and homeopathy. It is called the "King of Medicines" in Tibet and is listed first in the Ayurvedic material medica because of its extraordinary power of wound healing and a wide spectrum of medicinal properties. T. chebula possesses antibacterial, antifungal, antiviral, antidiabetic, antimutagenic, antioxidant, antiulcer and wound healing properties. It also prevents cardiac damage and is used for the treatment of kidney disease. It is a mild, safe and effective laxative in traditional medicine. T. chebula and its phytoconstituents have therapeutic effect with no toxicity. T. chebula is an active ingredient of the well known herbal preparation, Triphala, which is used for the treatment of enlarged liver, stomach disorders and pain in eyes. This review gives a bird's eye view on the biological and pharmacological properties of various extracts and isolated phytoconstituents of T. chebula to enrich our knowledge about this plant.
    No preview · Article · Jan 2012 · International Journal of Pharmacy and Pharmaceutical Sciences
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    ABSTRACT: Abstract Context: Arthritis is inflammation of one or more joints. Terminalia chebula Retz. (Combretaceae) fruit is mentioned in Ayurveda as useful in treating arthritic disorders. Objective: This work was undertaken to evaluate the anti-inflammatory, antioxidant, anti-lipid peroxidative and membrane-stabilizing effects of hydroalcoholic extract of Terminalia chebula fruits and also to establish a possible association between them. Materials and methods: In vivo anti-inflammatory activity of T. chebula fruit extract at different doses ranged from 50 to 500 mg/kg, p.o. was evaluated against carrageenin-induced inflammation in rats. Human erythrocyte hemolytic assay was used for in vitro anti-inflammatory activity testing with 50 to 500 µg/ml fruit extract. Antioxidant potential of test fruit extract (10 to 100 µg/ml) was evaluated using TBARS and DPPH methods. The fruit extract was standardized for total phenolic content using Folin-Ciocalteu method. Results: The standardized extract at 250 mg/kg, p.o. dose caused 69.96% reduction in carrageenin-induced rat paw edema and demonstrated 96.72% protective effect on human RBC membrane stability. Besides, T. chebula fruit extract significantly reduced the in vivo formation of TBARS in carrageenin-induced rat liver with IC50 94.96 mg/kg, p.o. and also in vitro radical scavenging activities in DPPH assay method with IC50 42.14 µg/ml. The standardized extract contains phenolics 118.5 mg gallic acid equivalent/g of extract. Discussion and conclusion: These promising findings support the traditional use of T. chebula fruits in the treatment of arthritic disorders and suggest that radical quenching may be one of the mechanisms for its anti-inflammatory activity.
    No preview · Article · Sep 2013 · Pharmaceutical Biology
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