Toward guided tissue and bone regeneration: Morphology, attachment, proliferation, and migration of cells cultured on collagen barrier membranes. A systematic review

Department of Periodontology and Biomaterials, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands.
Odontology (Impact Factor: 1.52). 07/2008; 96(1):1-11. DOI: 10.1007/s10266-008-0087-y
Source: PubMed


Collagen barrier membranes are frequently used in both guided tissue regeneration (GTR) and guided bone regeneration (GBR). Collagen used for these devices is available from different species and is often processed to alter the properties of the final product. This is necessary because unprocessed collagen is rapidly resorbed in vivo and demands for barrier membranes are different in GTR and GBR. This systematic literature review attempts to evaluate possible effects of collagen origin and mode of cross-linking on the potential of different cells to attach to, proliferate on, and migrate over barrier membranes in vitro. Seventeen original studies, selected by a systematic process, are included in this review. The results show that fibroblasts of different species and originating tissues as well as bone-forming cells are able to attach to collagen membranes irrespective of collagen origin or mode of processing. Different cell types behave differently on identical membranes. Many pieces of evidence are currently available, and we attempted to elucidate the effects of collagen origin and mode of processing on cellular behavior, but further research will be required before it will be possible to predict for certain the effect a specific procedure will have with a given product.

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    • "Collagen type III is also found in the dermis of skin [10] and is primarily responsible for tensile strength and accounts for about 20% of collagen increasing by 50% in healing wounds [13]. Previously, collagens were thought to function only as a structural support; however, it is now evident that collagen and collagen-derived fragments control many cellular functions, including cell shape and differentiation, 2,3 migration, 4 and synthesis of a number of proteins [14]. "
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    ABSTRACT: Australian native plants have a long history of therapeutic use in indigenous cultures, however, they have been poorly studied scientifically. We analysed the effects of 14 plant derived compounds from the species Pilidiostigma glabrum, Myoporum montanum, Geijera parviflora, and Rhodomyrtus psidioides for their potential wound healing properties by assessing their ability to induce or suppress Collagen I and Collagen III expression in human skin fibroblasts in culture. The compound 7-geranyloxycoumarin was able to significantly increase Collagen I (23.7%, p<0.0002) expression in comparison to control. Significant suppression of Collagen III was observed for the compounds flindersine (11.1%, p<0.02), and (N-acetoxymethyl) flindersine (27%, p<0.00005). The implications of these finding is that these compounds could potentially alter the expression of different collagens in the skin allowing for the potential development of new wound healing therapies and new approaches for treating various skin diseases as well as photo (sun) damaged, and aged skin.
    No preview · Article · Mar 2016 · Fitoterapia
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    • "Once cells are inserted, growth may be able to continue as normal in the tissue.25–28 However, defects that limit the application of collagen include its rapid degradation, lack of mechanical properties, and poor stability.29 To overcome these problems, we introduced two further constituents, ie, genipin for crosslinking collagen and PCL to reform the properties of the scaffold. "
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    • "It plays critical roles in many supporting and connecting tissues such as tendon, ligament, bone, blood vessels, skin, etc. Collagen gel prepared from commercially available collagen solution have been broadly used as a biomaterial in tissue engineering, drug delivery, and wound healing for its biocompatibility, low toxicity, and well-documented physical, chemical, and immunological properties.1-3 Collagen gel is also used as three-dimensional model systems of extracellular matrix (ECM) in numerous studies of cell-ECM interactions under physiological and pathological conditions.4-7 Collagen thin film, or dehydrated collagen gel, has been used as a two-dimensional platform in a number of studies to examine cell-ECM interactions.8 "
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