Qualitative and quantitative analysis of Radix Astragali products by fast high-performance liquid chromatography-diode array detection coupled with time-of-flight mass spectrometry through dynamic adjustment of fragmentor voltage

Key Laboratory of Modern Chinese Medicines, China Pharmaceutical University, Ministry of Education, Nanjing 210009, China.
Journal of Chromatography A (Impact Factor: 4.17). 08/2008; 1203(1):27-35. DOI: 10.1016/j.chroma.2008.07.019
Source: PubMed


A novel fast high-performance liquid chromatography (HPLC) method coupled with diode array detection (DAD) and time-of-flight mass spectrometry (TOF/MS) was developed for qualitative and quantitative analysis of Radix Astragali products. The potential of fast HPLC on 1.8-microm particles was compared with the performance of HPLC on conventional 5-microm particles columns. Significant advantages of fast HPLC include high-speed chromatographic separation, four times faster than HPLC with conventional columns, and great enhancement in sensitivity with limits of detection low to 0.001 ng. With dynamic adjustment of fragmentor voltage in TOF/MS, an efficient transmission of the ions was achieved to obtain the best sensitivity and abundant fragmentation. By accurate mass measurements within 5 ppm error for each molecular ion and subsequent fragment ions, a reliable identification and differentiation of six major saponins including two groups of isomers and twelve main isoflavonoids was described here for the first time. For quantitative analysis by fast HPLC-TOF/MS, linearity of response over two orders of magnitude was demonstrated (r(2)>0.99) for all analytes. Intra-day reproducibility was below 3% RSD and inter-day values were below 5% RSD. A good correlation (slope=1.1108, r(2)=0.9853) was observed for accuracy test. It is concluded that the fast and sensitive HPLC-DAD-TOF/MS is powerful in qualitative and quantitative analysis of complex herbal medicines in terms of time savings, sensitivity, selectivity, precision, accuracy as well as increasing sample throughout and lowering solvent consumption.

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Available from: Jun Cao, Apr 23, 2015
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    • "Recently, the rapid-resolution and ultra-performance liquid chromatography (RRLC/UPLC) methods have been established in many laboratories for qualitative and quantitative determinations of active components in TCM materials and preparations (Liu et al., 2010). In contrast to the conventional high performance liquid chromatography (HPLC), the proposed method is an attractive method for analysis, because the improved resolution, shorter retention times, low consumption of harmful organic solvent, higher sensitivity and better performance could be achieved for certain herbal medicines by using RRLC or UPLC (Liang et al., 2010;Qi et al., 2008) . To the best of our knowledge, there were no methods reporting the RRLC determination of organic acids and iridoid glycosides in reduning coupled to UV detection in literatures. "

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    • "Radix Astragali products are widely used not only as efficacious 44 medicinal prescriptions, but also as healthy food supplements (Qi 45 et al., 2008). It has been used for over 2000 years in traditional Chi- 46 nese medicine (TCM) prescriptions for the treatment of nephritis, 47 diabetes, albuminuria, hypertension, cirrhosis, cancer, as well as 48 a sedative and tonic (Li et al., 2001; Wang, Li, Yu, Zhao, & Ding, 49 2004). "
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