Royal Jelly Peptides Inhibit Lipid Peroxidation In Vitro and In Vivo

ArticleinJournal of Nutritional Science and Vitaminology 54(3):191-5 · June 2008with29 Reads
DOI: 10.3177/jnsv.54.191 · Source: PubMed
Abstract
Royal jelly peptides (RJPx) isolated from hydrolysates of water-soluble royal jelly proteins prepared with protease P exhibited significantly stronger hydroxyl radical-scavenging activity (p<0.001), and antioxidant activity against lipid peroxidation (LPO, p<0.001), than did water-soluble royal jelly protein (WSRJP) in vitro. We also investigated the in vivo antioxidant activity of RJPx against ferric nitrilotriacetate (Fe-NTA)-induced LPO. Male Wistar rats were divided into a control group (Group C), an Fe-NTA group (Group Fe), and an Fe-NTA with RJPx group (Group Fe+R). Rats in Group Fe+R were fed RJPx (2 g/kg body weight) daily for 5 wk. Fe-NTA (8 mg Fe/kg body weight) was then intraperitoneally injected, and serum lipid levels were examined 2 h later. Serum total cholesterol (TC) levels were lower (p<0.05) while low-density lipoprotein (LDL) and LPO were significantly higher (p<0.01) in Group Fe than in Group C. TC (p<0.05) and LPO levels (p<0.01) were lower in Group Fe+R than in Group Fe. Our data suggest that RJPx may inhibit LPO both in vitro and in vivo.
    • "Besides antitumor, antihypertensive, antiinflammatory and antiallergenic effects, cell proliferative effects of RJ were also reported (GUO et al., 2008). RJ is rich in vitamins, especially water-soluble vitamins (vitamins B and C) and minerals such as potassium, calcium, magnesium, iron, zinc, sulfur and copper (STOCKER et al., 2005; GUO et al., 2008). In a previous study MOGHADDAM et al. (2014) reported the improving effects of royal jelly on hatching rate and embryonic growth of broiler chicks. "
    Full-text · Article · Jan 2016
    • "The dried powders were stored at À20 C in darkness. The extracts were prepared using the methods described by Nagai and Inoue [21] and Guo et al [22] with modifications. Three grams of leaf and stem powders was suspended and extracted with 30 volumes of 10 mM of sodium phosphate buffer (pH 7.0) then shaken at 4 C for 24 hours. "
    [Show abstract] [Hide abstract] ABSTRACT: In this study, leaves and stems of Talinum triangulare were sequentially extracted with phosphate buffer solution to obtain PTL and PTS (phosphate buffered extracts of T. triangulare leaves and stems), with 75% ethanol to obtained ETL and ETS (ethanol extracts of T. triangulare leaves and stems), or with 90°C boiling water to obtain WTL and WTS (water extracts of T. triangulare leaves and stems). We investigated the antioxidant activities of various T. triangulare extracts, analyzed the extracts' stimulations on human mononuclear cell (MNC) growth and secretion of cytokines (interleukin-1 beta, interferon-γ, and tumor necrosis factor-α) and nitric oxide, and then assayed their subsequent inhibitions on human leukemic U937 cell growth. Results indicated that extracts of T. triangulare showed significant antioxidant activities. Among these extracts, WTS showed the highest stimulatory effect on human MNC growth. The secretion levels of interleukin-1 beta, interferon-γ, and tumor necrosis factor-α in the conditioned medium, wherein human MNC was treated with 500 μg/mL WTS for 72 hours, were 1275, 859, and 2222 pg/mL, respectively. All conditioned media obtained from human MNCs cultured with various T. triangulare extracts showed significant inhibition against U937 cell growth of over 40%. These results suggest that T. triangulare extracts may be used in health foods for their immunomodulatory potential.
    Full-text · Article · Jan 2015
    • "According to Pizzorno et al. (2007), RJ is efficient to protect DNA against oxidative damage. It has also been shown to inhibit lipid peroxidation both in vitro and in vivo (Hang et al., 2008). When mice are fed RJ for 16 wk, the levels of 8-hydroxy-2-deoxyguanosine (an oxidative stress marker) were significantly reduced in kidney, in the same row having different superscripts are significantly different (P<0.05). "
    [Show abstract] [Hide abstract] ABSTRACT: To alleviate the deleterious effect of heat stress during summer conditions on male rabbits' reproduction, 40 V Line adult rabbit bucks (on av. 8 mo old) were divided into 4 experimental groups and exposed to temperatures ranging from 23 to 36° C. Bucks in the 1st, 2nd, 3rd, and 4thgroup were supplemented with 0, 50, 100 or 150 mg of Chinese royal jelly (RJ)/kg twice per week, respectively, over a 20-wk period. Semen quality and blood biochemical constituents were evaluated. RJ at any dose exhibited a significant increase (P<0.05) in rabbits' sperm concentration, total sperm output, sperm motility, live sperm and normal sperm compared to the untreated controls. Plasma total protein, albumin, globulin, glucose and high density lipids (HDL) concentrations were significantly (P<0.05) boosted in the RJ groups compared to the controls. In contrast, RJ treatment resulted in a significant (P<0.05) reduction in plasma total lipids, triglycerides, cholesterol and low density lipids (LDL) concentrations. Treatment with RJ significantly boosted (P<0.05) testosterone concentration in the RJ groups to reach 110, 120 and 128%, respectively, of the control group. Improved kidney and liver functions were observed in the RJ bucks groups where plasma creatinine, urea concentrations, aspartate aminotransferase and alanine aminotransferase enzyme activities were significantly (P<0.05) decreased by RJ treatments. Treating bucks subjected to heat stress by different RJ doses increased (P<0.05) total antioxidant capacity to 106, 111 and 115% of basal, but significantly reduced (P<0.05) malondialdehyde and thiobarbituric acid reactive substances compared to the untreated. It was concluded that Chinese royal jelly supplementation for heat-stressed male rabbits can counteract summer infertility and improve their physiological status.
    Article · Sep 2014
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