Article

Caenorhabditis elegans Teneurin, ten-1, Is Required for Gonadal and Pharyngeal Basement Membrane Integrity and Acts Redundantly with Integrin ina-1 and Dystroglycan dgn-1

Friedrich Miescher Institute for Biomedical Research, Novartis Research Foundation, CH-4058 Basel, Switzerland.
Molecular biology of the cell (Impact Factor: 4.47). 09/2008; 19(9):3898-908. DOI: 10.1091/mbc.E08-01-0028
Source: PubMed

ABSTRACT

The Caenorhabditis elegans teneurin ortholog, ten-1, plays an important role in gonad and pharynx development. We found that lack of TEN-1 does not affect germline proliferation but leads to local basement membrane deficiency and early gonad disruption. Teneurin is expressed in the somatic precursor cells of the gonad that appear to be crucial for gonad epithelialization and basement membrane integrity. Ten-1 null mutants also arrest as L1 larvae with malformed pharynges and disorganized pharyngeal basement membranes. The pleiotropic phenotype of ten-1 mutant worms is similar to defects found in basement membrane receptor mutants ina-1 and dgn-1 as well as in the mutants of the extracellular matrix component laminin, epi-1. We show that the ten-1 mutation is synthetic lethal with mutations of genes encoding basement membrane components and receptors due to pharyngeal or hypodermal defects. This indicates that TEN-1 could act redundantly with integrin INA-1, dystroglycan DGN-1, and laminin EPI-1 in C. elegans development. Moreover, ten-1 deletion sensitizes worms to loss of nidogen nid-1 causing a pharynx unattached phenotype in ten-1;nid-1 double mutants. We conclude that TEN-1 is important for basement membrane maintenance and/or adhesion in particular organs and affects the function of somatic gonad precursor cells.

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    • "e not muscle - specific ( Henry and Campbell , 1996 ) . Dystroglycan plays an important role in kidney epithelial morphogenesis by its interaction with laminin ( Henry and Campbell , 1996 ) , as well ; neuronal dystro - glycan is responsible for the maintenance of Schwann cell myeli - nation and axonal guidance ( Masaki and Matsumura , 2010 ) . In Trzebiatowska et al . ( 2008 ) initially reported an association of the teneurins with dystroglycans . It was concluded that the teneurins have partly related and redundant functions in C . ele - gans development , particularly with respect to the organization and maintenance of the basement membrane in tissues associ - ated with the gonad , pharynx and hypodermis "
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