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Pseudomonas duriflava sp nov., isolated from a desert soil

Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.51). 06/2008; 58(Pt 6):1404-8. DOI: 10.1099/ijs.0.65716-0
Source: PubMed

ABSTRACT

A Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium, designated strain HR2(T), was isolated from a soil sample from the Taklimaken Desert in Xinjiang Province, China. Strain HR2(T) grew optimally at pH 7.0-8.0 and 30-37 degrees C in the presence of 0-1 % (w/v) NaCl. An analysis of 16S rRNA gene sequences revealed that strain HR2(T) fell within the radiation of the genus Pseudomonas, the highest level of similarity being found with respect to Pseudomonas luteola IAM 13000(T) (97.5 %); the levels of sequence similarity with respect to other recognized Pseudomonas species were <96.4 %. DNA-DNA hybridization showed that the genetic relatedness between strain HR2(T) and P. luteola IAM 13000(T) was 53.2 %. The G+C content of the genomic DNA of strain HR2(T) was 55.2 mol%. The major fatty acids were 18 : 1, summed feature 3 and 16 : 0. The hydroxylated fatty acids 10 : 0 3-OH, 12 : 0 3-OH and 12 : 0 2-OH were also present. The data obtained in this polyphasic study indicated that this isolate represents a novel species of the genus Pseudomonas, for which the name Pseudomonas duriflava sp. nov. is proposed. The type strain is HR2(T) (=KCTC 22129(T)=CGMCC 1.6858(T)).

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Available from: Chongxing Zhang, Jan 07, 2015
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    • "The genus Pseudomonas includes more than 100 species, a number that is increasing in time [2]. Nearly each year, a new species is indeed discovered, like P. duriflava, P. batumici or P. litoralis for example, isolated from a desert soil [3], the Caucasus Black sea coast [4] or from Mediterranean seawater [5], respectively. Due to its heterogeneity, the genus Pseudomonas has undergone numerous taxonomic changes depending on the criteria employed for their definition and delineation: phenotypic, physiologic or metabolic characteristics, siderotyping, phylogeny based on 16S rRNA and/or “housekeeping” genes, analysis of 16S-23S rRNA intergenic spacers (ITS) or the use of functional and ecological genetic markers such as oprF, oprD or gacA[2,6-8]. "
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    ABSTRACT: Background The genus Pseudomonas includes a heterogeneous set of microorganisms that can be isolated from many different niches and nearly 100 different strains have been described. The best characterized bacterium is Pseudomonas aeruginosa which is the primary agent of opportunistic infection in humans, causing both acute and chronic infections. Other species like fluorescens, putida or mosselii have been sporadically isolated from hospitalized patients but their association with the pathology often remains unclear. Results This study focuses on the cytotoxicity and inflammatory potential of two strains of Pseudomonas mosselii (ATCC BAA-99 and MFY161) that were recently isolated from clinical samples of hospitalized patients. The behavior of these bacteria was compared to that of the well-known opportunistic pathogen P. aeruginosa PAO1. We found that P. mosselii ATCC BAA-99 and MFY161 are cytotoxic towards Caco-2/TC7 cells, have low invasive capacity, induce secretion of human β-defensin 2 (HBD-2), alter the epithelial permeability of differentiated cells and damage the F-actin cytoskeleton. Conclusions These data bring new insights into P. mosselii virulence, since this bacterium has often been neglected due to its rare occurrence in hospital.
    Full-text · Article · May 2013 · BMC Microbiology
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    • "Strain BY-1 T was isolated from an agricultural field, which had been exposed to the herbicide fomesafen for many years, by using the standard dilution plating technique on Luria–Bertani (LB) agar (5 g yeast extract, 10 g tryptone, 10 g NaCl; pH 7.0) followed by incubation for 2 days at 30 u C under aerobic conditions. The isolate was routinely cultivated on LB agar or in LB broth at the same temperature, and was maintained in a glycerol suspension (20 %, v/v) at 270 u C. Pseudomonas luteola IAM 13000 T (Kodama et al., 1985) and Pseudomonas duriflava HR2 T (Liu et al., 2008) were employed as controls in phenotypic tests. Unless otherwise mentioned, these strains were grown aerobically on TSA or in TSB at 30 u C. "
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    ABSTRACT: A Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium, designated strain BY-1T, was isolated from a soil sample from the city of Qiqihar in Heilongjiang Province, China. Strain BY-1T grew optimally at pH 7.0 and 30-35°C in the presence of 0.5% (w/v) NaCl. An analysis of 16S rRNA gene sequences revealed that strain BY-1T fell within the radiation of the genus Pseudomonas, as it showed highest sequence similarities to Pseudomonas luteola IAM 13000T (99.5 %) and Pseudomonas duriflava HR2T (97.3 %); the levels of sequence similarity with respect to other recognized Pseudomonas species were < 96.7 %. Strain BY-1T showed low DNA-DNA relatedness values with P. luteola IAM 13000T(29±3.1%) Pseudomonas duriflava HR2T (21±1.5 %). The G+C content of the genomic DNA of strain BY-1T was 55.3 mol%. The major fatty acids were C18:1, C16:0, and Summed features 3 (C16:1ω6c and/or C161ω7c). Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major ubiquinone was Q-9. The data obtained in this polyphasic study indicated that this isolate represents a novel species of the genus Pseudomonas, for which the name Pseudomonas zeshuii sp. nov. is proposed. The type strain is BY-1T (= KACC 15471T= ACCC 05688T).
    Preview · Article · Dec 2011 · International Journal of Systematic and Evolutionary Microbiology
    • "Other species have been isolated from plant phyllosphere, as P. lurida from grass phyllosphere (Behrendt et al., 2007). Some species have been isolated from marine environments such as P. marincola from marine ecosystems (Romanenko et al., 2008) and several species were isolated from extreme environments such as P. duriflava from desert soil (Liu et al., 2008), P. guineae, a psychrotolerant bacterium, from Antarctic soil (Bozal et al., 2007), P. psychrotolerans isolated in a veterinary clinic (Hauser et al., 2004) or P. thermotolerans, which can grow at 55 8C and was isolated from animal clinical samples (Manaia and Moore, 2002). This worldwide distribution seems to be due to a high genetic and physiological adaptability (Spiers et al., 2000) being the presence of numerous genomic islands in the genus a key to the adaptability traits of individual strains to such ubiquitous environments, for example in the strain P. aeruginosa PSE9 (Battle et al., 2009). "
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    ABSTRACT: The genus Pseudomonas was described in 1894 and is one of the most diverse and ubiquitous bacterial genera whose species have been isolated worldwide in all kinds of environments, from the Antarctica to the Tropics, present in sediments, clinical samples, plant, fungi and diseased animal specimens, water, soil, plant rhizosphere, sea, deserts, etc. The taxonomy of the genus has been controversial for years since a lot of bacterial taxa initially included in genus Pseudomonas have been reclassified in other genera or species from a different class of Proteobacteria over the years, as techniques for characterization and classification of microorganisms improved, aiming to set a phylogenetic classification of the species. In this review, the historical evolution of the taxonomy of Pseudomonas is described, and the currently valid criteria and future challenges for taxonomy of the genus and techniques used to achieve the necessary characterization for classifying the species are discussed. Finally, all the validly published Pseudomonas species at present are listed with an overview of their diversity and ecology.
    No preview · Article · Sep 2009 · Infection, genetics and evolution: journal of molecular epidemiology and evolutionary genetics in infectious diseases
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