Cytogenetic and DNA-Fingerprint Characterization of Choriocarcinoma Cell Lines and a Trophoblast /Choriocarcinoma Cell Hybrid

ArticleinCancer Genetics and Cytogenetics 116(1):16-22 · January 2000with5 Reads
Impact Factor: 1.93 · DOI: 10.1016/S0165-4608(99)00107-7 · Source: PubMed


    We report the successful fusion of human choriocarcinoma cells with normal human trophoblast cells to a choriocarcinoma/trophoblast hybrid. The hybrid cells ACH1P were derived from fusion of primary male trophoblast cells with the HGPRT-defective choriocarcinoma cell line AC1-1. The karyotypes of the parental choriocarcinoma cell line JEG-3, its HGPRT-defective mutant clones AC1-1, AC1-5, and AC1-9, and the choriocarcinoma/trophoblast hybrid ACH1P are presented, together with a detailed characterization of the AC1-specific chromosomal marker add(X)(q26) using conventional cytogenetic banding techniques and multiplex-fluorescence in situ hybridization (M-FISH). To our knowledge, this is the first report of a stably proliferating human cell hybrid of trophoblastic origin, providing a unique cell culture model to study trophoblast-related invasion and its underlying genetic mechanisms.