Human platelet antigen frequencies of platelet donors in the French population determined by polymerase chain reaction with sequence-specific primers

ArticleinPathologie Biologie 45(9):697-700 · November 1997with3 Reads
Source: PubMed
To prevent human platelet alloimmunization, Blood Transfusion Centres have to develop a strategy close to the erythrocytes' one. The first step of this strategy is to perform the HPA typing of donors with an accurate method. We applied the PCR-SSP to type 800 platelet donors in the HPA-1 and HPA-5 systems and 350 in the HPA-2 and HPA-3 ones. This study reports the human platelet antigen frequencies of four platelet-specific alloantigen systems in the French population. The results are quite similar to those currently published for Caucasian population frequencies. Low prevalences are observed for the HPA-1b, (2%), HPA-2b (0.6%) and HPA-5b (2%) groups. Furthermore, this study confirms the need to type donors and recipients in the HPA-1 system at least, in case of post-transfusion pupura and platelet refractoriness to platelet transfusion therapy.
    • "Besides, HPA-1b allele is significantly higher in Saudis than in black Brazilians (Castro et al., 1999); otherwise, the rest of the HPA systems findings among Saudis and black Brazilians were similar. As for the Far Eastern populations, in White Australians (Bennett et al., 2002), and like other Europeans (see above),Boehlen et al., 2003) 500 80·9 19·1 91·8 10·9 59·1 40·7 99·7 0·3 93·4 6·6 NA NA NA NA French (Merieux et al., 1997) 800 84·8 15·2 92 8 62 38 NA NA 87·4 12·6 NA NA 45·5 54·5 America Brazilian, black (Castro et al., 1999) 150 90·3 9·7 81 19 66·6 33·4 100 0 87·6 12·4 NA NA NA NA Argentinian (De La Vega et al., 2008) 192 87·8 12·2 87·5 12·5 61·2 38·8 100 0 92·7 7·3 100 0 51·1 48·9 Brazilian, white (Castro et al., 1999 NA indicates that gene frequencies were not available. the only notable significant difference, the distribution of HPA alleles between them and Saudis, is observed in the distribution of the HPA-2 and -5b that is higher in Saudis (P = 0·0001) (P = 0·015). "
    [Show abstract] [Hide abstract] ABSTRACT: Background: Human platelet antigens (HPAs) are involved in the pathogenesis of several clinical conditions, such as platelet transfusion purpura (PTP), refractoriness to platelet transfusion and neonatal alloimmune thrombocytopenia (NAITP). Typing of HPA (1-6 and 15) has not been carried on the Saudi population. This is the first study of all the seven HPA systems on Arabs. The aim of this prospective study was to determine the frequency of HPA (1-6 and 15) in Saudis. Study design and methods: A total of 100 randomly selected Saudi blood donor samples were genotyped using the polymerase chain reaction with sequence-specific primers (PCR-SSP). Results: The most common HPA genotypes among Saudis were HPA-1 a + b- (75%), HPA-2 a + b- (62%), HPA-3 a + b- (51·5%), HPA-4 a + b- (99%), HPA-5 a + b- (76·5%), HPA-6 a + b- (100%) and HPA-15 a + b + (50%). The prevalent allele among the HPA systems was (a), except in the HPA-15 system where the (b) allele was found in 52% of the subjects. Comparisons with other ethnic populations uncovered marked differences in the distribution of HPA alleles. Conclusion: Studying the prevalence of HPA antigens in Saudi population will help in the understanding of its role in platelet-related disorders. It will also enable the blood bank to establish an HPA-based donor registry that will be a valuable source of compatible platelet-therapeutic products to alloimmunised patients. This will also enhance the safety and efficacy of platelet transfusion. This data obtained will form an addition to the existing body of literature in transfusion research.
    Full-text · Article · Mar 2016
    • "Our Caucasian group was selected for these comparisons (genetic aspects) because it was most tive of the population of Rio Grande do Sul, and because it accounted for the majority of our sample (83%). No significant differences in allele frequencies were observed between our Caucasian group and the populations of Argentina (De La Vega Elena et al., 2008; HPA-1 to 5 and 15), Italy (EBI, 2014; HPA-1 to 5), Germany (EBI, 2014; HPA-1 to 3 and 5), Croatia (Pavic et al., 2010; HPA-1 to 3 and 5), the United Kingdom (Jones et al., 2003; HPA-1 to 5), Slovenia (Rozman et al., 1999; HPA-1 to 5), France (Merieux et al., 1997; HPA-1 to 5), Denmark (Steffensen et al., 1996; HPA-1 to 5), Austria (Holensteiner et al., 1995; HPA-1 to 3 and 5), Poland (Drzewek et al., 1998; HPA-1 to 5) or Spain (Muniz-Diaz et al., 1993 ; HPA- 2 to 5 and 15). For HPA-1, we found a significant difference between our sample and the Spanish population (HPA-1a = 0.867 and 1b = 0.133 vs. HPA- 1a = 0.810 and 1b = 0.190, respectively, P < 0.05). "
    [Show abstract] [Hide abstract] ABSTRACT: Human platelet antigens (HPA) are immunogenic structures that result from single nucleotide polymorphisms (SNPs) leading to single amino acid substitutions. This study sought to determine the allele and genotype frequencies of HPA-1, HPA-2, HPA-3, HPA-4, HPA-5 and HPA-15 in platelet donors from the state of Rio Grande do Sul (RS), Brazil, and compare their allele frequencies to those observed in other populations. HPA genotyping was performed by PCR-SSP method. The study sample comprised 201 platelet donors (167 Caucasians and 34 non-Caucasians). Allele 'a' was that most commonly found for HPA-1 to 5 in both groups. The HPA-15ab genotype predominated over homozygous genotypes of this system. Fisher's exact test revealed statistically significant differences for the HPA-5 system, with a greater prevalence of the HPA-5b allele in non-Caucasians. The neighbour-joining method and principal components analysis revealed genetic proximity between our Caucasian group and European populations. We conclude that the allele frequencies of HPA-1 to 5 and HPA-15 found in our Caucasian sample are similar to those reported for European populations. These findings corroborate the ethnic makeup of the population of RS. The higher frequency of the HPA-5b allele found in the non-Caucasian group of our sample suggests the possibility of allosensitization in patients who receive platelet transfusions from genetically incompatible donors. © 2015 John Wiley & Sons Ltd.
    Article · Jul 2015
    • "There are no previous reports of the genotypes and allele frequencies of HPA genes in an Egyptian Arabic population, but studies from many other countries are reported: i.e. Morocco [22], Tunisia [23], China (Han) [24], sub-Saharan Africans [25], Czech Republic [26], Denmark [27], France [28], Germany [29,30] and Algeria [31]. The genotype frequencies of HPA-1-5 obtained in the study of the Egyptian Arabic population differ from the genotype frequencies of Caucasian and Chinese (Han) populations, and are not significantly different to the reported frequencies in studies from Tunisia and Morocco. "
    [Show abstract] [Hide abstract] ABSTRACT: BACKGROUND AND OBJECTIVES: Foetal and neonatal alloimmune thrombocytopenia (FNAIT) is studied mainly in Caucasian populations. Severe thrombocytopenia (<50×10(9)/L) gives risk of haemorrhage and the most feared complication is intracranial haemorrhage (ICH). In Caucasian populations anti-human platelet antigen (HPA)-1a antibodies are the cause of FNAIT in >80% of the cases. The aims of this project were to study the gene frequencies of HPA-1-5 and 15 alleles in an Egyptian population (Arabic), and to determine the frequency of HPA-1a and -5b immunisations in a cohort of Egyptian pregnant women. MATERIALS AND METHODS: Altogether 6974 pregnant women were included in the study. Genotyping was performed by polymerase chain reaction and antibodies were detected by flow cytometry and enzyme-linked immunosorbent assay. HPA-1-5 and 15 alleles were studied in 367 individuals. RESULTS: The HPA genotypes differed from genotypes published from different Caucasian and Chinese (Han) populations in HPA-1, -2, -3, and -5 systems with significant higher frequency of HPA-1b, -2b and -5b. The rate of HPA-1a alloimmunisation was found comparable to Caucasian populations. Severe thrombocytopenia was found in two newborns. No bleeding complication was reported. Anti-HPA-5b antibodies were detected in 4.4% of the pregnant women. Clinical consequences of these antibodies were not studied. CONCLUSION: The HPA-1bb and -5bb genotypes are more frequent in the Egyptian Arabic population studied compared to Caucasian populations. FNAIT due to anti-HPA-1a and -5b antibodies must be suspected in cases of neonatal thrombocytopenia. Further large prospective studies are needed to increase the knowledge of clinical complications related to HPA alloantibodies in populations with different genetic backgrounds.
    Full-text · Article · May 2012
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