Lack of nucleotide variability in a beetle pest with extreme breeding

Department of Entomology, University of Wisconsin-Madison, USA.
Insect Molecular Biology (Impact Factor: 2.59). 05/1998; 7(2):197-200. DOI: 10.1046/j.1365-2583.1998.72064.x
Source: PubMed


The coffee berry borer beetle Hypothenemus hampei (Ferrari) (Curculionidae: Scolytinae) is the major insect pest of coffee and has spread to most of the coffee-growing countries of the world. This beetle also displays an unusual life cycle, with regular sibling mating. This regular inbreeding and the population bottlenecks occurring on colonization of new regions should lead to low levels of genetic diversity. We were therefore interested in determining the level of nucleotide variation in nuclear and mitochondrial genomes of this beetle worldwide. Here we show that two nuclear loci (Resistance to dieldrin and ITS2) are completely invariant, whereas some variability is maintained at a mitochondrial locus (COI), probably corresponding to a higher mutation rate in the mitochondrial genome. Phylogenetic analysis of the mitochondrial data shows only two clades of beetle haplotypes outside of Kenya, the proposed origin of the species. These data confirm that inbreeding greatly reduces nucleotide variation and suggest the recent global spread of only two inbreeding lines of this bark beetle.

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    • "Those molecular techniques and others like PCR amplification followed by restriction endonuclease analysis (PCR–REN) [10] [19] and real-time PCR application using TaqMan [20] have been successfully developed to detect the resistance-associated mutation in other insect species. The Rdl gene was the main genetic marker used previously to study population genetic, heredity and reproductive behavior of the coffee berry borer [21] [22] [23], but it was not found before in the Colombian H. hampei insect popula- 0048-3575/$ -see front matter Ó 2010 Elsevier Inc. All rights reserved. "
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    • "These cyclical bottlenecks could have also erased or modified ancestral polymorphism. Such lack of information might explain the inability to resolve basal relationships among clades, as well as the incongruence between Cyt-b and COI markers indicated by the ILD test (Andreev et al., 1998; Gandolfi et al., 2001; Gorog et al., 2004). 4.3. "
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    • "Any of these hypotheses might apply to all permanently inbred social systems such as spiders, psocids, naked mole rats, and some bark beetles (e.g. Mockford 1957; New 1985; Sherman et al. 1991; Kirkendall 1993; O'Riain et al. 1996; Andreev et al. 1998; Burland et al. 2002). Susceptibility to pathogens and inability to respond to changing environments (e.g., Slate and Pemberton 2002; Day et al. 2003; Schön et al. 2003; Pujolar et al. 2005) seem particularly relevant for social spiders due to their strong population subdivision, inbreeding , and high rates of colony turnover (Avilés 1993, 1997), all of which might erode genetic variability (Smith and Hagen 1996; Johannesen et al. 2002). "
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