HIV Type 1 Mutational Patterns in HIV Type 1 Subtype C-Infected Long-Term Survivors in Karonga District Malawi: Further Analysis and Correction

Molecular Evolution and Systematics Laboratory, Zoology, Ryan Institute, School of Natural Sciences, National University of Ireland, Galway, Ireland.
AIDS research and human retroviruses (Impact Factor: 2.33). 06/2011; 28(3):308-13. DOI: 10.1089/AID.2011.0168
Source: PubMed


Here we present new sequence data from HIV-1 subtype C-infected long-term survivors (LTS) from Karonga District, Malawi. Gag and env sequence data were produced from nine individuals each of whom has been HIV-1 positive for more than 20 years. We show that the three amino acid deletion in gag p17 previously described from these LTS is not real and was a result of an alignment error. We find that the use of dried blood spots for DNA-based studies is limited after storage for 20 years. We also show some unlikely amino acid changes in env C2-V3 in LTS over time and different patterns of genetic divergence among LTS. Although no clear association between mutations and survival could be shown, amino acid changes that are present in more than one LTS may, in the future, be shown to be important.

Download full-text


Available from: Grace Mccormack, Jan 07, 2014