Green synthesis and characterization of gelatin-based and sugar-reduced silver nanoparticles

Advanced Materials and Nanotechnology Laboratory, Institute of Advanced Technology (ITMA), Universiti Putra Malaysia, Selangor, Malaysia.
International Journal of Nanomedicine (Impact Factor: 4.38). 03/2011; 6(1):569-74. DOI: 10.2147/IJN.S16867
Source: PubMed


Silver nanoparticles (Ag-NPs) have been successfully prepared with simple and "green" synthesis method by reducing Ag(+) ions in aqueous gelatin media with and in the absence of glucose as a reducing agent. In this study, gelatin was used for the first time as a reducing and stabilizing agent. The effect of temperature on particle size of Ag-NPs was also studied. It was found that with increasing temperature the size of nanoparticles is decreased. It was found that the particle size of Ag-NPs obtained in gelatin solutions is smaller than in gelatin-glucose solutions, which can be related to the rate of reduction reaction. X-ray diffraction, ultraviolet-visible spectra, transmission electron microscopy, and atomic force microscopy revealed the formation of monodispersed Ag-NPs with a narrow particle size distribution.

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    • "Taking the principles of green chemistry into account, it was reported that for the synthesis of Ag-NPs they have used starch as stabilizing M A N U S C R I P T A C C E P T E D ACCEPTED MANUSCRIPT agent[18]and glucose as a reducing agent[16]and both of which are non-toxic, environmentally safe and renewable materials. Several other similar works have been reported which used nontoxic materials such as gelatin and maltose[19,20]and also the use of plant extracts in the synthesis for Ag-NPs.[21,22]. Here we report the use of carboxymethyl sago pulp obtained from sago waste for the synthesis of Ag-NPs via EB radiation. "
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    ABSTRACT: Green synthesis of silver nanoparticles (Ag-NPs) via electron beam (EB) radiation using carboxymethyl sago pulp (CMSP) was achieved. The conditions for the synthesis of CMSP capped silver nanoparticles were optimized by varying the pH, concentration of CMSP, degree of substitution of CMSP (DS), EB radiation dose and the size of the nanoparticles were studied. UV–vis spectroscopy shows the maximum surface plasmon resonance (SPR) peak (nm) decreased as the pH increased indicating that an increase in the pH of the solution favored the formation of smaller particles. The FTIR spectra confirmed the capping of Ag-NPs with CMSP. The XRD peaks showed Braggs reflections of silver revealing the face centered cubic structure of Ag-NPs. The TEM micrographs showed that the Ag-NPs were well dispersed and spherical in shape. The average particle mean diameters were calculated to 15.00 ± 1.07 nm for 1% CMSP (DS 0.8), pH 11 and 50 kGy sample which was in close resemblance with the DLS average particle size, 16.00 ± 0.30 nm. The green synthesis of Ag-NPs embedded in CMSP hydrogel is a highly clean material that is expected to be economical and competent antimicrobial materials in biomedical fields.
    Full-text · Article · Jan 2016
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    • "Gelatin as an important protein has a three-chain helical structure and it contains positively and negatively charged and it can stabilize surfaces by the formation of a steric barrier [23]. "
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    ABSTRACT: The use of biopolymers in the synthesis of different nanostructures can be a cost effective and eco-friendly approach. In the present study, a facile and "green" sol-gel method was employed for preparing calcium hydroxide nanoparticles (Ca(OH)2-NPs) in gelatin matrix as a bio-template. Prepared nanoparticles were characterized by different instruments such as powder X-ray diffraction (PXRD), field emission scanning electron microscopy (FESEM), and Fourier transform infrared spectroscopy (FTIR). The PXRD analysis revealed hexagonal Ca(OH)2-NPs with preferential orientation in (101) reflection plane. They are hexagonal in shape with a mean particle size of approximately 42nm in thickness. The synthesized Ca(OH)2-NPs using gelatin were found to be comparable to those obtained from conventional methods using hazardous capping/stabilizing polymeric agents or surfactants and this route can be an excellent alternative for the synthesis of Ca(OH)2-NPs using biomaterials.
    Full-text · Article · Nov 2015
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    • "Therefore, it is essential to seek a simple route for low-cost, large-scale, controlled growth of CeO 2 -NPs at atmospheric pressure and lower temperatures. Lately, many researchers have used natural and organic matrix components such as gelatin, starch, gums, etcetera, in the synthesis of different nanoparticles since they are considered capable of controlling polymorphism, morphology , and the size of nanoparticles [20] [21] [22]. Recently, natural and organic matrices have been used in the synthesis of CeO 2 -NPs [23] [24] [25], due to their quality of being biodegradable and bio-absorbable with degradation products that are non-toxic. "
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    ABSTRACT: The use of bio-based materials such as food-directed products for the synthesis of different nanoparticles (e.g., metal and metal oxide) is of enormous interest to modern nanoscience and nanotechnology. We have developed a simple and green chemistry method with bio-directed, available, and low cost materials for the synthesis of size-controlled ceria nanopowders (CeO2-NPs) using fresh egg white (EW), as an eco-friendly foamy substrate. The proteins of EW, which have various functional groups, played important roles in the synthesis of CeO2-NPs as capping and stabilizing agents. The synthesized CeO2-NPs were characterized by the following title: UV-vis spectroscopy, field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric (TGA-DTA) analysis, and powder X-ray diffraction (PXRD). Spherical CeO2-NPs were synthesized at different calcination temperatures (200, 400, 600, 800 °C) and FESEM imaging along with its corresponding particles size distribution indicated the formation of nanopowders in size of about 25 nm. The PXRD analysis revealed fluorite cubic structure for CeO2-NPs with preferential orientation at (1 1 1) reflection plane. In vitro cytotoxicity studies on human periodontal fibroblasts cells showed a dose dependent toxicity with non-toxic effect of concentration up to 800 μg/ml. The synthesis of CeO2-NPs in EW was found to be comparable to those obtained from conventional preparation methods that uses hazardous materials proving to be an excellent alternative for the preparation of CeO2-NPs, using food and bio-derived materials.
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