Deep Sequencing Reveals Novel MicroRNAs and Regulation of MicroRNA Expression during Cell Senescence

Center for Genetics, Children's Hospital Oakland Research Institute, Oakland, California, United States of America.
PLoS ONE (Impact Factor: 3.23). 05/2011; 6(5):e20509. DOI: 10.1371/journal.pone.0020509
Source: PubMed


In cell senescence, cultured cells cease proliferating and acquire aberrant gene expression patterns. MicroRNAs (miRNAs) modulate gene expression through translational repression or mRNA degradation and have been implicated in senescence. We used deep sequencing to carry out a comprehensive survey of miRNA expression and involvement in cell senescence. Informatic analysis of small RNA sequence datasets from young and senescent IMR90 human fibroblasts identifies many miRNAs that are regulated (either up or down) with cell senescence. Comparison with mRNA expression profiles reveals potential mRNA targets of these senescence-regulated miRNAs. The target mRNAs are enriched for genes involved in biological processes associated with cell senescence. This result greatly extends existing information on the role of miRNAs in cell senescence and is consistent with miRNAs having a causal role in the process.

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Available from: David I K Martin
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    • "The SA-miRNAs control cell transition , mainly through the G 1 /S checkpoint during cell cycle progression by targeting the components of cell cycle including cyclin-dependent kinases (CDKs) and cyclin-dependent kinase inhibitors (CDKIs) [16]. Despite the reported discrepancies between the upregulation and downregulation of miRNAs during aging and cellular senescence, such as miR-34a [17] [18], study of the modulatory effect of dietary compound on miRNAs may aid in the understanding of how SA-miRNAs can be regulated in favour of slowing down aging process or reducing aging phenotypes. Modulation of miRNAs by dietary and pharmacological agents has been reported recently [19]. "
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    • "Furthermore, epigenetically, microRNAs (miRNA) could influence many basic cellular and pathological processes by regulating gene expression post-transcriptionally through binding to complementary sequences in the 3′ untranslated region (3′ UTR) of target mRNAs [16]. And recent studies showed that miRNAs participated in regulation of aging and a variety of age-associated pathways [17]. "
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    • "Increasing evidence has confirmed that miRNAs act as novel cellular senescence regulators [26,27]. However, there is little information regarding the potential involvement of miRNAs in regulating the cellular senescence of the corneal endothelium. "
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