Unusual Genotype of a Uropathogenic Escherichia coli Strain Assigned to the B2 Phylogenetic Group

Center of Pharmaceutical Sciences, Faculty of Pharmacy, University of Coimbra, Coimbra, Portugal.
Journal of clinical microbiology (Impact Factor: 3.99). 06/2011; 49(8):3105-6. DOI: 10.1128/JCM.00585-11
Source: PubMed


Extraintestinal pathogenic Escherichia coli (ExPEC) cause infections such as urinary tract infections, septicemia and meningitis.…

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Available from: Nuno Mendonça, Jul 23, 2014
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    • "In order to determine the impact of the mutations we compared Doumith's multiplex PCR protocol [5] updating the design of the primers used in the 2000 Clermont's method [4], reducing unspecific annealing and promoting specific amplification, thus improving coverage. Non-specific amplifications (as for an acetyl-hydrolase gene) using conventional Clermont's protocol have been reported for B1 isolates [22], but this protocol has also occasionally failed in the amplification of chuA and/or yjaA [5], [23], [24], [25], [26]. In fact, in our series Clermont's multiplex PCR approach provides a worse assignment (14.0% "
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